Epigenetic silencing of JAM3 promotes esophageal cancer development by activating Wnt signaling

Autor:	Weili Yang, Chao Guo, James G. Herman, Cheng Zhu, Honghui Lv, Xiaomo Su, Lirong Zhang, Meiying Zhang, Mingzhou Guo
Rok vydání:	2022
Předmět:	Esophageal Neoplasms Immunoglobulins DNA Methylation Decitabine Epigenesis Genetic Mice Genetics Animals Humans Molecular Biology Cell Adhesion Molecules Protein Processing Post-Translational Wnt Signaling Pathway Genetics (clinical) Developmental Biology
Zdroj:	Clinical epigenetics. 14(1)
ISSN:	1868-7083
Popis:	BackgroundThe role of JAM3 in different tumors is controversial. The epigenetic regulation and the mechanism of JAM3 remain to be elucidated in human esophageal cancer (EC).MethodsEleven EC cell lines, 49 cases of esophageal intraepithelial neoplasia (EIN) and 760 cases of primary EC samples were employed. Methylation-specific polymerase chain reaction, immunohistochemistry, MTT, western blot and xenograft mouse models were applied in this study.ResultsThe inverse association between RNA expression and promoter region methylation of JAM3 was found by analyzing 185 cases of EC samples extracted from the TCGA database (p p p ConclusionJAM3 is frequently methylated in human EC, and the expression of JAM3 is regulated by promoter region methylation. JAM3 methylation is an early detection and prognostic marker of EC. JAM3 suppresses EC growth both in vitro and in vivo by inhibiting Wnt signaling.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd3c2671de05cf7baf5ce10db21d855c https://pubmed.ncbi.nlm.nih.gov/36461092 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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