Homocysteine Hypothesis on the Impaired Peripheral but Not Central Nervous System Oxytocin Responses in Cystathionine γ-Lyase-Deficient Dam Mice
Autor: | Waka Kamichatani, Noriyuki Akahoshi, Isao Ishii |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Homocysteine Mammary gland Hyperhomocysteinemia Pharmaceutical Science Oxytocin Blood–brain barrier Mice 03 medical and health sciences chemistry.chemical_compound Mammary Glands Animal 0302 clinical medicine Limit of Detection Pregnancy Internal medicine Lactation Peripheral Nervous System Citrulline Animals Humans Medicine Milk Ejection Maternal Behavior Mice Knockout Pharmacology Behavior Animal biology business.industry Cystathionine gamma-Lyase General Medicine Ornithine Cystathionine beta synthase Disease Models Animal 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Blood-Brain Barrier 030220 oncology & carcinogenesis biology.protein Female business medicine.drug |
Zdroj: | Biological and Pharmaceutical Bulletin. 43:1810-1813 |
ISSN: | 1347-5215 0918-6158 |
Popis: | An elevated plasma homocysteine level is an independent risk factor for cardiovascular diseases, neurological disorders, and pregnancy complications. We recently demonstrated partial lactation failure in cystathionine γ-lyase-deficient (Cth-/-) dam mice and their defective oxytocin responses in peripheral tissues: uterine (ex vivo) and mammary gland (in vivo). We reasoned that elevated levels of circulatory homocysteine in Cth-/- dam mice counteract with oxytocin-dependent milk ejection from the mammary gland. Based on our observation that those mice displayed normal maternal behaviors against their pups and adult Cth-/- male mice exhibited normal social behaviors against adult wild-type female mice, both of which are regulated by oxytocin in the central nervous system (CNS), we conducted the present study to investigate the amino acid profiles, including total homocysteine, in both blood and cerebrospinal fluid (CSF) of wild-type and Cth-/- female mice before pregnancy and at day 1 of lactation (L1). Serum levels of total homocysteine in wild-type and Cth-/- L1 dam mice were 9.44 and 188 µmol/L, respectively, whereas their CSF levels were below 0.21 (limit of quantification) and 3.62 µmol/L, respectively. Their CSF/serum level ratio was the lowest (1/51.9) among all 20 proteinogenic amino acids, sulfur-containing amino acids, and citrulline/ornithine in Cth-/- mice. Therefore, we hypothesize that the blood-brain barrier protects the CNS from high levels of circulatory homocysteine in Cth-/- dam mice, thereby conferring normal oxytocin-dependent maternal behaviors. |
Databáze: | OpenAIRE |
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