The major excretory/secretory protease from Lucilia cuprina larvae is also a gut digestive protease
| Autor: | C.H. Eisemann, Ross L. Tellam, Tony Vuocolo, Rosanne E. Casu |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
animal structures medicine.medical_treatment Immunoblotting Polymerase Chain Reaction digestive system Microbiology Internal medicine parasitic diseases medicine Animals Chymotrypsin Tissue Distribution Secretion Calliphoridae Sheep Protease biology Diptera fungi Hindgut Midgut medicine.disease biology.organism_classification Infectious Diseases Endocrinology Lucilia cuprina Larva Digestive enzyme biology.protein Insect Proteins Parasitology Myiasis Digestive System |
| Zdroj: | International Journal for Parasitology. 26:623-628 |
| ISSN: | 0020-7519 |
| DOI: | 10.1016/0020-7519(96)00035-5 |
| Popis: | The larvae of the fly Lucilia cuprina excrete or secrete a chymotrypsia (LCTb) onto the skin of sheep to facilitate the establishment of the larval infestation. A combination of immunoblotting and RT-PCR approaches has established that this protease is also a gut digestive protease. LCTb is synthesized primarily in the cardia, a small highly specialized organ located at the anterior end of the midgut and by midgut cells. There is also some expression by the hindgut but no expression by salivary glands. Excretion of LCTb with waste products or regurgitation of the gut contents of the larvae may explain how this protease is transferred from the larval gut onto ovine skin. LCTb is first expressed in eggs and constitutively expressed throughout each larval instar, but is not expressed in pupae or adult flies. It is concluded that LCTb could be involved in the establishment of larvae on sheep skin as well as acting as a general gut digestive enzyme. |
| Databáze: | OpenAIRE |
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