Apoptosis and reduced influenza A virus specific CD8+ T cells in aging mice
| Autor: | P Tebebi, I N Mbawuike, X Gilmore, Ying Wang, Keyi Xu, Yongxin Zhang, M Chen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Aging
Fas Ligand Protein Lymphocyte T cell Apoptosis Biology medicine.disease_cause Fas ligand Interferon-gamma Mice Adjuvants Immunologic medicine Influenza A virus Cytotoxic T cell Animals fas Receptor Molecular Biology Membrane Glycoproteins hemic and immune systems Cell Biology Molecular biology Interleukin-12 CTL medicine.anatomical_structure Immunologic Memory CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Cell death and differentiation. 9(6) |
| ISSN: | 1350-9047 |
| Popis: | Some studies have reported increased apoptosis in CD8(+) T cells from aged mice. We previously demonstrated diminished virus-specific CD8(+) cytotoxic T lymphocyte (CTL) activity in aged mice in comparison to young mice. The present study investigated the role of apoptosis in age-related influenza virus-specific CD8(+) CTL deficiency. Splenocytes from influenza-primed aged and young mice were stimulated in vitro with virus. The CD8(+) T cell/total lymphocyte ratios correlated with CTL activity and were significantly decreased and increased in aged and young mice, respectively. Fas, FasL, TNF-alpha and TNFR-p55 expression, measured by flow cytometry, ELISA and/or RT-PCR, were significantly elevated in aged mice. Apoptotic CD8(+) T cells (Annexin V binding) were also elevated in aged mice. IL-12 treatment increased CD8(+) CTL activity and IFN-gamma production but did not affect apoptosis. Thus, apoptosis may contribute to reduced influenza virus-specific CD8(+) T cell frequency, CTL deficiency and increased influenza disease in aging. |
| Databáze: | OpenAIRE |
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