Free Choline, but Not Phosphatidylcholine, Elevates Circulating Trimethylamine-N-oxide and This Response Is Modified by the Gut Microbiota Composition in Healthy Men
| Autor: | Michael Lefevre, Niklas D J Aardema, Deanna Larson, Madison L Bunnell, Sheryl S. Aguilar, Janet R Bergeson, Clara E. Cho, Marie A. Caudill, Olga V. Malysheva |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Nutrition and Dietetics food.ingredient biology Medicine (miscellaneous) Trimethylamine Trimethylamine N-oxide Dietary Bioactive Components Gut flora biology.organism_classification Lecithin chemistry.chemical_compound Endocrinology food Clostridium chemistry Phosphatidylcholine Internal medicine medicine Choline Microbiome Food Science |
| Zdroj: | Curr Dev Nutr |
| Popis: | OBJECTIVES: Trimethylamine-N-oxide (TMAO), a choline-derived gut microbiota-dependent metabolite, is a newly recognized risk marker for cardiovascular disease. However, the contributions of different forms of choline and gut microbiota composition on TMAO production are largely unknown. The objectives of this study were to: 1) compare acute TMAO response to meals containing free choline (choline bitartrate) versus fat-soluble choline (phosphatidylcholine) and 2) to determine the effects of gut microbiota composition on TMAO response. METHODS: In a controlled, double-blinded, cross-over study, healthy men (n = 37) were provided meals containing (i) 600 mg choline as choline bitartrate (free choline); (ii) 600 mg choline as phosphatidylcholine; or (iii) no choline control in a random order. Blood and urine samples were collected at baseline and throughout the 6-h study period; a one-time stool sample was collected at baseline. RESULTS: Compared to no choline and phosphatidylcholine, free choline yielded 295% higher plasma TMAO (P = 0.002) and 250% higher urinary TMAO (P = 0.01), with no difference in TMAO response between phosphatidylcholine and no choline. High-TMAO producers (those with ≥40% increase in urinary TMAO response to free choline) had significantly different beta-diversity measures (unweighted UniFrac; PERMANOVA P = 0.01) compared to low-TMAO producers (those with |
| Databáze: | OpenAIRE |
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