Reduction of Tendon Adhesions following Administration of Adaprev, a Hypertonic Solution of Mannose-6-Phosphate: Mechanism of Action Studies

Autor: Richard J. Wong, Duncan A. McGrouther, Jim Bush, Arnaud Garcon, Nick Goldspink, Mark W. J. Ferguson, Chris Platt, Anthony D. Metcalfe, Jason Wong
Rok vydání: 2014
Předmět:
Pathology
Critical Care and Emergency Medicine
Hypertonic Solutions
lcsh:Medicine
Tissue Adhesions
Tendon Injuries/drug therapy
Mice
Transforming Growth Factor beta
Signal Transduction/drug effects
Medicine and Health Sciences
Medicine
Biomechanics
Muscle Components
lcsh:Science
Musculoskeletal System
Wound Healing/drug effects
Trauma Medicine
Cells
Cultured
Mannosephosphates
Tensile Strength/drug effects
Multidisciplinary
Hand Strength
Muscles
Cell migration
Animal Models
musculoskeletal system
Biomechanical Phenomena
Cell biology
Tendon
medicine.anatomical_structure
Research Design
Collagen
Rabbits
Anatomy
Plastic Surgery and Reconstructive Techniques
Transforming Growth Factor beta/metabolism
Research Article
Signal Transduction
Cell Physiology
medicine.medical_specialty
Clinical Research Design
Trauma Surgery
Surgical and Invasive Medical Procedures
Mouse Models
Research and Analysis Methods
Fibroblast migration
Model Organisms
Tendon Injuries
In vivo
Tensile Strength
Collagen/metabolism
Animals
Humans
Animal Models of Disease
Viability assay
Tissue Adhesions/metabolism
Fibroblast
Mode of action
Cell Proliferation
Mannosephosphates/administration & dosage
Wound Healing
business.industry
lcsh:R
Biology and Life Sciences
Cell Biology
Disease Models
Animal
Animal Studies
lcsh:Q
business
Ex vivo
Zdroj: Wong, J K F, Metcalfe, A D, Wong, R, Bush, J, Platt, C, Garcon, A, Goldspink, N, McGrouther, D A & Ferguson, M W J 2014, ' Reduction of tendon adhesions following administration of Adaprev, a hypertonic solution of mannose-6-phosphate : Mechanism of action studies ', PLoS ONE, vol. 9, no. 11, e112672 . https://doi.org/10.1371/journal.pone.0112672
PLoS ONE, Vol 9, Iss 11, p e112672 (2014)
Wong, J K F, Metcalfe, A D, Wong, R, Bush, J, Platt, C, Garcon, A, Goldspink, N, McGrouther, D A & Ferguson, M W J 2014, ' Reduction of tendon adhesions following administration of Adaprev, a hypertonic solution of mannose-6-phosphate : Mechanism of action studies ', P L o S One, vol. 9, no. 11, e112672 . https://doi.org/10.1371/journal.pone.0112672
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0112672
Popis: Repaired tendons may be complicated by progressive fibrosis, causing adhesion formation or tendon softening leading to tendon rupture and subsequent reduced range of motion. There are few therapies available which improve the gliding of damaged tendons in the hand. We investigate the role of Mannose 6-phosphate (M6P) in a 600 mM hypertonic solution (Adaprev) on tendon adhesion formation in vivo using a mouse model of severed tendon in conjunction with analysis of collagen synthesis, cellular proliferation and receptors involved in TGF beta signalling. Cytotoxicity was assessed by measuring tissue residency, mechanical strength and cell viability of tendons after treatment with Adaprev. To elicit potential modes of action, in vitro and ex vivo studies were performed investigating phosphorylation of p38, cell migration and proliferation. Adaprev treatment significantly (p
Databáze: OpenAIRE
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