Suppression of temperature-sensitive sporulation mutation in theBacillus subtilis sigAgene byrpoBmutation
| Autor: | Roy H. Doi, Hideaki Nanamiya, Kei Asai, Nobutake Fugono, Fujio Kawamura |
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| Rok vydání: | 2000 |
| Předmět: |
Hot Temperature
Transcription Genetic Blotting Western Mutant Sigma Factor Bacillus subtilis Microbiology chemistry.chemical_compound Suppression Genetic Transcription (biology) Sigma factor RNA polymerase Genetics Molecular Biology Suppressor mutation Spores Bacterial biology fungi DNA-Directed RNA Polymerases biology.organism_classification Temperature-sensitive mutant rpoB Molecular biology Amino Acid Substitution chemistry Mutation Transcription Factors |
| Zdroj: | FEMS Microbiology Letters. 192:237-241 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2000.tb09388.x |
| Popis: | We isolated a temperature-sensitive sporulation defective mutant of the sigA gene, encoding a major sigma factor, sigma(A) protein, in Bacillus subtilis, and designated it as sigA21. The sigA21 mutation caused a single-amino acid substitution, E314K, in region 4 of the sigma(A) protein. In this mutant, expression of the spoIIG gene, whose transcription depends on both sigma(A) and the phosphorylated Spo0A protein, Spo0A approximately P, a major transcription factor during early stages of sporulation, was greatly reduced at 43 degrees C. To obtain further information on the mechanism of sigma(A) function during the early spore development, we isolated a spontaneous sporulation-proficient suppressor mutant at 43 degrees C. This extragenic suppressor mutation was mapped within the rpoB gene, encoding the beta subunit of RNA polymerase, and was found to have a single-amino acid substitution, A863G. In this mutant, the expression of the spoIIG is partially restored at 43 degrees C. |
| Databáze: | OpenAIRE |
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