Haemin-binding proteins of Porphyromonas gingivalis W50 grown in a chemostat under haemin-limitation
| Autor: | Ailsa S. McKee, Andrew J. Birss, John W. Smalley, Philip Marsh |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Hemeproteins
Biological Transport Active Microbiology Cofactor Cell wall Heme-Binding Proteins Cell Wall Cell surface receptor Mole Porphyromonas gingivalis Gel electrophoresis Staining and Labeling biology Chemistry Benzidines biology.organism_classification Molecular biology Biochemistry biology.protein Hemin Electrophoresis Polyacrylamide Gel Carrier Proteins Bacterial outer membrane Bacteria Bacterial Outer Membrane Proteins |
| Zdroj: | Journal of General Microbiology. 139:2145-2150 |
| ISSN: | 0022-1287 |
| DOI: | 10.1099/00221287-139-9-2145 |
| Popis: | Porphyromonas gingivalis W50 was grown in a chemostat at pH 7.3 under haemin-limitation and haemin-excess at a constant mean doubling time of 6.9 h. Outer membranes (OM) were extracted from whole cells using EDTA and compared by SDS-PAGE. Haemin-limited cells expressed novel outer membrane proteins (OMPs) of mol. mass 115, 113 and 19 kDa when samples were solubilized at 100 degrees C. A 46 kDa OMP was observed in haemin-excess cells but not in those from haemin-limited conditions. Tetramethylbenzidine (TMBZ) staining of gels, after OM solubilization at 20 degrees C, was used to detect haemin-binding proteins (HBPs). HBPs were observed only in OM from haemin-limited cells. The major HBP (mol. mass 32.4 kDa) corresponded to a similar sized Kenacid-blue-stained protein which was not observed in haemin-excess-derived OM. Haemin-limited cells and OM displayed a ladder-like series of Kenacid-blue-stained proteins. Lighter TMBZ-stained proteins of mol. mass 51, 53, 56 and 60 kDa, with mobilities corresponding to those of silver-stained LPS components, were observed in haemin-limited OM. No soluble HBPs were detected extracellularly. The greater number of HBPs expressed by cells grown under haemin-limitation may reflect an additional cell surface receptor system for haemin acquisition under low environmental levels of this essential cofactor. |
| Databáze: | OpenAIRE |
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