Adipocyte deficiency of ACE2 increases systolic blood pressures of obese female C57BL/6 mice
| Autor: | Charles Mark Ensor, Wen Su, Lisa R. Tannock, Frederique Yiannikouris, Robin Shoemaker, Lisa A. Cassis, Susan B. Gurley, Ming Gong, Sean E. Thatcher |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty medicine.drug_class Adipose tissue lcsh:Medicine Peptidyl-Dipeptidase A Diet High-Fat lcsh:Physiology Gender Studies 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Endocrinology Internal medicine Adipocyte Renin–angiotensin system medicine Adipocytes Animals Obesity Sex Characteristics lcsh:QP1-981 business.industry Research lcsh:R Angiotensin-converting enzyme 2 Angiotensin-(1-7) medicine.disease Angiotensin II Peptide Fragments Menopause 030104 developmental biology Blood pressure chemistry Gene Expression Regulation Estrogen Transgender Female Angiotensin I business 030217 neurology & neurosurgery hormones hormone substitutes and hormone antagonists |
| Zdroj: | Biology of Sex Differences, Vol 10, Iss 1, Pp 1-12 (2019) Biology of Sex Differences |
| ISSN: | 2042-6410 |
| DOI: | 10.1186/s13293-019-0260-8 |
| Popis: | Background Obesity increases the risk for hypertension in both sexes, but the prevalence of hypertension is lower in females than in males until menopause, despite a higher prevalence of obesity in females. We previously demonstrated that angiotensin-converting enzyme 2 (ACE2), which cleaves the vasoconstrictor, angiotensin II (AngII), to generate the vasodilator, angiotensin-(1-7) (Ang-(1-7)), contributes to sex differences in obesity-hypertension. ACE2 expression in adipose tissue was influenced by obesity in a sex-specific manner, with elevated ACE2 expression in obese female mice. Moreover, estrogen stimulated adipose ACE2 expression and reduced obesity-hypertension in females. In this study, we hypothesized that deficiency of adipocyte ACE2 contributes to obesity-hypertension of females. Methods We generated a mouse model of adipocyte ACE2 deficiency. Male and female mice with adipocyte ACE2 deficiency or littermate controls were fed a low (LF) or a high fat (HF) diet for 16 weeks and blood pressure was quantified by radiotelemetry. HF-fed mice of each sex and genotype were challenged by an acute AngII injection, and blood pressure response was quantified. To translate these findings to humans, we performed a proof-of-principle study in obese transwomen in which systemic angiotensin peptides and blood pressure were quantified prior to and after 12 weeks of gender-affirming 17β-estradiol hormone therapy. Results Adipocyte ACE2 deficiency had no effect on the development of obesity in either sex. HF feeding increased systolic blood pressures (SBP) of wild-type male and female mice compared to LF-fed controls. Adipocyte ACE2 deficiency augmented obesity-induced elevations in SBP in females, but not in males. Obese female, but not obese male mice with adipocyte ACE2 deficiency, had an augmented SBP response to acute AngII challenge. In humans, plasma 17β-estradiol concentrations increased in obese transwomen administered 17β-estradiol and correlated positively with plasma Ang-(1-7)/AngII balance, and negatively to SBP after 12 weeks of 17β-estradiol administration. Conclusions Adipocyte ACE2 protects female mice from obesity-hypertension, and reduces the blood pressure response to systemic AngII. In obese transwomen undergoing gender-affirming hormone therapy, 17β-estradiol administration may regulate blood pressure via the Ang-(1-7)/AngII balance. Electronic supplementary material The online version of this article (10.1186/s13293-019-0260-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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