Microbiota‐Dependent Involvement of Th17 Cells in Murine Models of Inflammatory Arthritis
Autor: | Heather L. Evans-Marin, Debbie M. Roeleveld, Shahla Abdollahi-Roodsaz, Jose U. Scher, Sergei B. Koralov, R. Rogier, Marije I. Koenders, Peter M. van der Kraan, Julia Manasson |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Inflammatory arthritis medicine.medical_treatment T cell Immunology Arthritis Rheumatoid Arthritis Flow cytometry Mice 03 medical and health sciences All institutes and research themes of the Radboud University Medical Center 0302 clinical medicine Rheumatology medicine Animals Immunology and Allergy Cytotoxic T cell Intestinal Mucosa Immunity Mucosal 030203 arthritis & rheumatology Lamina propria medicine.diagnostic_test business.industry medicine.disease Arthritis Experimental Gastrointestinal Microbiome Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cytokine Cytokines Th17 Cells Original Article Tumor necrosis factor alpha business Inflammatory diseases Radboud Institute for Molecular Life Sciences [Radboudumc 5] |
Zdroj: | Arthritis & Rheumatology, 70, 12, pp. 1971-1983 Arthritis & Rheumatology (Hoboken, N.j.) Arthritis & Rheumatology, 70, 1971-1983 |
ISSN: | 2326-5205 2326-5191 |
Popis: | Objective Intestinal microbiota are associated with the development of inflammatory arthritis. The aim of this study was to dissect intestinal mucosal immune responses in the preclinical phase of arthritis and determine whether the presence of Th17 cells, beyond involvement of the cytokine interleukin-17 (IL-17), is required for arthritis development, and whether the involvement of Th17 cells in arthritis depends on the composition of the host microbiota. Methods Mucosal T cell production of IL-17, interferon-γ, tumor necrosis factor α (TNFα), IL-22, and granulocyte-macrophage colony-stimulating factor (GM-CSF) was analyzed by flow cytometry and Luminex assay before arthritis onset in mice immunized to develop collagen-induced arthritis (CIA). Pathogenic features of arthritis in mice with CIA and mice with antigen-induced arthritis were compared between Th17 cell-deficient (CD4-Cre+ Rorcflox/flox ) and Th17 cell-sufficient (CD4-Cre- Rorcflox/flox ) mice. In addition, the impact of intestinal microbiota on the Th17 cell dependence of CIA was assessed. Results Lamina propria CD4 T cells were activated before the onset of arthritis in mice with CIA, with marked up-regulation of several cytokines, including IL-17A, TNFα, and GM-CSF. CD4-Cre+ Rorcflox/flox mice showed a specific reduction in intestinal mucosal levels of Th17 cells and partially reduced levels of IL-17-producing CD8 T cells. However, total levels of IL-17A, mostly produced by γδ T cells and neutrophils, were unaffected. The severity of arthritis was significantly reduced in Th17 cell-deficient mice, suggesting that Th17 cells have additional, IL-17A-independent roles in inflammatory arthritis. Accordingly, antigen-stimulated T cells from Th17 cell-deficient mice produced less IL-17A, IL-17F, and GM-CSF. Importantly, the dependence of CIA on the involvement of Th17 cells was mitigated in the presence of an alternative microbiome. Conclusion These data from murine models suggest that activation of mucosal immunity precedes the development of arthritis, and also that Th17 cells have a microbiota-dependent role in arthritis. Therefore, a microbiome-guided stratification of patients might improve the efficacy of Th17-targeted therapies. |
Databáze: | OpenAIRE |
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