Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix
| Autor: | Cristina Takami Kanamura, Marina Suheko Oyafuso, Jeni Bortolan, Venancio Avancini Ferreira Alves, Marina Yoshiê Sakamoto Maeda, Adhemar Longatto Filho |
|---|---|
| Přispěvatelé: | Adolfo Lutz Institute, A. C. Camargo Hospital, Universidade Estadual Paulista (UNESP) |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Histology Cervix neoplasms Uterine Cervical Neoplasms Cervix Uteri Pathology and Forensic Medicine Biopsy medicine Carcinoma Humans Cervix neoplasm Papillomaviridae Aged Vaginal Smears medicine.diagnostic_test Radiotherapy business.industry Papillomavirus Infections HPV infection Cancer General Medicine Middle Aged Papillomavirus medicine.disease Immunohistochemistry Koilocyte Tumor Virus Infections Epidermoid carcinoma Cytopathology Female business Human |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2022-04-28T19:59:54Z (GMT). No. of bitstreams: 0 Previous issue date: 1997-01-01 OBJECTIVE: To report the cytomorphologic evidence of human papillomavirus (HPV) infection in cervical smears from women treated with radiotherapy for carcinoma of the uterine cervix. STUDY DESIGN: From January 1986 to December 1993, 32 cervical cytologic smears (CCSs) from women who had received radiotherapy (Rtx) for squamous cell cervical carcinoma at A. C. CamargoCancer Hospital were selected for study due to the presence of signs of HPV infection. Review of the files of these patients showed 22 additional samples, which we included in this study. The avidinbiotin- immunoperoxidase technique was performed on previously stained slides using polyclonal rabbit antibovine papillomavirus type 1 to confirm the HPV infection. RESULTS: Positive reactions were found in 18 cases (27 samples), mainly in cells with classic koilocytotic features, but also in epithelial cells that lacked cytopathic effects. Only one case, a biopsy from a verrucous lesion observed after Rtx, was found to be positive for HPV 6/11 DNA by in situ hybridization. CONCLUSION: Cytologic and immunohistochemical positivity for HPV, as well as the reaction with a HPV 6/11 DNA probe, strongly suggested that HPV infection was acquired or expressed after radiotherapy. Further studies are needed to assess the mechanisms responsible forsuch an association. © 1997 The International Academy of Cytology Acta Cytologica. Pathology Division Adolfo Lutz Institute Pathology Department A. C. Camargo Hospital Department of Pathology São Paulo State University Medical School, São Paulo A. C. Camargo Hospital Adolfo Lutz Institute Adolfo Lutz Institute São Paulo State University Medical School A. C. Camargo Hospital Department of Pathology São Paulo State University Medical School, São Paulo Adolfo Lutz Institute São Paulo State University Medical School |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|