Immobilization of heparin/poly-l-lysine microspheres on medical grade high nitrogen nickel-free austenitic stainless steel surface to improve the biocompatibility and suppress thrombosis

Autor: Wang Guixue, Xiaojuan Zhang, Xiaoling Liao, Hans Gregersen, Tieying Yin, Haide Wu, Yi Wang, Menghua Li
Rok vydání: 2016
Předmět:
medicine.medical_treatment
Dopamine
Biocompatible Materials
Cell Count
02 engineering and technology
Antioxidants
Restenosis
Nickel
Polylysine
Austenitic stainless steel
medicine.diagnostic_test
Cell Death
Antithrombin
Heparin
021001 nanoscience & nanotechnology
Microspheres
Mechanics of Materials
Partial Thromboplastin Time
Rabbits
0210 nano-technology
medicine.drug
Partial thromboplastin time
Dental Alloys
Blood Platelets
Materials science
Biocompatibility
Nitrogen
Surface Properties
0206 medical engineering
Static Electricity
Bioengineering
Nanotechnology
Thrombin time
engineering.material
Nitric Oxide
Hemolysis
Biomaterials
Platelet Adhesiveness
medicine
Human Umbilical Vein Endothelial Cells
Animals
Humans
Particle Size
Cell Shape
Cell Proliferation
Stent
Fibrinogen
Thrombosis
medicine.disease
Stainless Steel
020601 biomedical engineering
engineering
Prothrombin Time
Biomedical engineering
Zdroj: Materials scienceengineering. C, Materials for biological applications. 73
ISSN: 1873-0191
Popis: Thrombosis formation, restenosis, and delayed endothelium regeneration continue to be a challenge for coronary artery stent therapy. To improve the hemocompatibility of cardiovascular implants and to selectively direct vascular cell behavior, a novel heparin/poly-l-lysine microsphere was developed and immobilized on a dopamine-coated surface. We chose medical grade high nitrogen nickel-free austenitic stainless steel as the stent material since it has better biocompatibility. The stability and structural characteristics of the microspheres changed with the heparin: poly-l-lysine concentration ratio. Antithrombin III binding was significantly enhanced. Furthermore, for plasma coagulation tests, the activated partial thromboplastin time and thrombin time were prolonged and depended on the heparinfunction. The modified exhibited excellent stability and anticoagulant activity, and efficiently accelerated endothelialization and anticoagulation. This work has potential application for the design of coronary artery stent surfaces tailored for vascular cell behavior.
Databáze: OpenAIRE
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