Intracellular mannose binding lectin mediates subcellular trafficking of HIV-1 gp120 in neurons
| Autor: | Benchawanna Soontornniyomkij, S. Divakar, Cristian L. Achim, Marcus Kaul, C. Teodorof, Kumud K. Singh |
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| Rok vydání: | 2014 |
| Předmět: |
viruses
Mannose Golgi Apparatus Mannose binding lectin HIV Envelope Protein gp120 Endoplasmic Reticulum Microtubules chemistry.chemical_compound 2.2 Factors relating to the physical environment Aetiology Cells Cultured Neuronal transport Microtubule associated protein-2 Neurons Microscopy HIV-1 gp120 Microscopy Confocal Cultured Tumor Blotting Nocodazole virus diseases Tubulin Modulators Cell biology Transport protein Protein Transport Infectious Diseases Neurology Confocal symbols HIV/AIDS Western Intracellular Cells Blotting Western Clinical Sciences chemical and pharmacologic phenomena Biology Mannose-Binding Lectin Article lcsh:RC321-571 Cell Line Subcellular trafficking symbols.namesake Microtubule Cell Line Tumor Neurites Humans Immunoprecipitation Transport Vesicles lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Organelles Neurology & Neurosurgery Endoplasmic reticulum Neurosciences Golgi apparatus bacterial infections and mycoses Good Health and Well Being chemistry HIV-1 |
| Zdroj: | Neurobiology of Disease, Vol 69, Iss, Pp 54-64 (2014) |
| Popis: | Human immunodeficiency virus -1 (HIV-1) enters the brain early during infection and leads to severe neuronal damage and central nervous system impairment. HIV-1 envelope glycoprotein 120 (gp120), a neurotoxin, undergoes intracellular trafficking and transport across neurons; however mechanisms of gp120 trafficking in neurons are unclear. Our results show that mannose binding lectin (MBL) that binds to the N-linked mannose residues on gp120, participates in intravesicular packaging of gp120 in neuronal subcellular organelles and also in subcellular trafficking of these vesicles in neuronal cells. Perinuclear MBL:gp120 vesicular complexes were observed and MBL facilitated the subcellular trafficking of gp120 via the endoplasmic reticulum (ER) and Golgi vesicles. The functional carbohydrate recognition domain of MBL was required for perinuclear organization, distribution and subcellular trafficking of MBL:gp120 vesicular complexes. Nocodazole, an agent that depolymerizes the microtubule network, abolished the trafficking of MBL:gp120 vesicles, suggesting that these vesicular complexes were transported along the microtubule network. Live cell imaging confirmed the association of the MBL:gp120 complexes with dynamic subcellular vesicles that underwent trafficking in neuronal soma and along the neurites. Thus, our findings suggest that intracellular MBL mediates subcellular trafficking and transport of viral glycoproteins in a microtubule-dependent mechanism in the neurons. |
| Databáze: | OpenAIRE |
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