Hepatic transcription response to high-fat treatment in mice: Microarray comparison of individual vs. pooled RNA samples
| Autor: | Myung-Sook Choi, Hyeng-Soo Kim, Eun-Young Kwon, Eun-Jung Kim, Gyeong-Min Do |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Research Report Messenger RNA Transcription Genetic Microarray analysis techniques Gene Expression Profiling RNA General Medicine Biology Dietary Fats Applied Microbiology and Biotechnology Molecular biology Fold change Mice Inbred C57BL Gene expression profiling Mice Liver Transcription (biology) Models Animal Gene expression Animals Molecular Medicine RNA Messenger Gene Oligonucleotide Array Sequence Analysis |
| Zdroj: | Biotechnology Journal. 5:970-973 |
| ISSN: | 1860-6768 |
| DOI: | 10.1002/biot.201000046 |
| Popis: | Microarray analysis is an important tool in studying gene expression profiles in genomic research. Despite many concerns raised, mRNA samples are often pooled in microarray experiments to reduce the cost and complexity of analysis of transcript profiling. This study reports the results of microarray experiments designed to compare effects of pooling RNA samples and its impact on identifying profiles of mRNA transcripts and differentially expressed genes (DEGs) in the liver of C57BL/6J mice fed normal and high-fat diet. Pearson's correlation coefficient of transcripts between pooled and non-pooled RNA samples was 0.98 to 1.0. The impact of pooled vs. non-pooled RNA samples was also compared by number of transcripts or DEGs. Agreement of significant genes between pooled and non-pooled sets was fairly desirable based on t-test |
| Databáze: | OpenAIRE |
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