Effects of inaccurate reference lifetimes on interpreting frequency-domain fluorescence data

Autor:	Jingfan Huang, Frank V. Bright, Kevin S. Litwiler
Rok vydání:	1990
Předmět:	business.industry Chemistry Reference Standards Analytical Chemistry Kinetics Spectrometry Fluorescence Software Optics Frequency domain fluorescence Statistical physics business Error detection and correction Phase modulation Reference standards
Zdroj:	Analytical Chemistry. 62:471-476
ISSN:	1520-6882 0003-2700
Popis:	The effects of assigning inaccurate reference lifetimes in lifetime determinations are predicted theoretically by using standard equations. This theory leads to a method to remove reference error effects using common least-squares software. This method cannot, however, be used to deconvolute data collected with isochronal references. Uncorrected data can always be exactly solved with models containing one more degree of freedom than the true model. Monoexponential decays are fit by double-exponential decays or excited-state processes. Unimodal distributed decays often appear as discrete, double-exponential decays.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd1bc8ca352fc3a0757a4d39bbc70460 https://doi.org/10.1021/ac00204a010 Zobrazit plný text záznamu