Effects of the Long Non-Coding RNA HOST2 On the Proliferation, Migration, Invasion and Apoptosis of Human Osteosarcoma Cells
| Autor: | Chun-Yang Yang, Wei Wang, Fan-bin Meng, Ren-Tao Zhao, Xiao Li, Zhen-xing Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Adolescent Physiology Cell Proliferation Bone Neoplasms Apoptosis lcsh:Physiology Flow cytometry lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine Invasion Cell Movement Cell Line Tumor medicine Humans lcsh:QD415-436 RNA Small Interfering Child Migration Cell Proliferation Osteosarcoma medicine.diagnostic_test lcsh:QP1-981 Cell growth Chemistry Cell migration Transfection Long non-coding RNA-HOST2 Cell cycle G1 Phase Cell Cycle Checkpoints Molecular biology 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Female RNA Interference RNA Long Noncoding |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 43, Iss 1, Pp 320-330 (2017) |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Background/Aims: This study aimed to explore the effects of the long non-coding RNA HOST2 (lnc-HOST2) on the proliferation, migration, invasion and apoptosis of osteosarcoma cells. Methods: Osteosarcoma tissues and adjacent normal tissues from 52 patients were selected. Human osteosarcoma cell lines (SaOS2, HOS, U2OS and MG-63) were collected and cultured; MG-63 cells had the highest lnc-HOST2 expression and thus were used in subsequent experiments. Then, MG-63 cells were transfected and divided into the blank (no transfection), si-CON (transfected with negative control siRNA) and si-lnc-HOST2 (transfected with small interference lnc-HOST2 siRNA) groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the expression of lnc-HOST2 in primary tissues and cells. Cell growth was detected using the CCK-8 and colony formation assays. Cell doubling time was detected. Cell migration and invasion were observed using the scratch test and Transwell assays. Cell apoptosis and cell cycle progression of osteosarcoma cells were detected using flow cytometry with annexin V/PI double staining and PI staining, respectively. Results: The level of lnc-HOST2 expression in the si-lnc-HOST2 group was significantly decreased compared to that in the blank and si-CON groups. The OD values in the si-lnc-HOST2 group were significantly lower than those in the blank and si-CON groups. Compared to the blank and si-CON groups, the si-lnc-HOST2 group presented significant decreases in the colony number and healing rates after scratching. The number of invasive cells in the si-lnc-HOST2 group was significantly less than that in the blank and si-CON groups. In the si-lnc-HOST2 group, the cell cycle was mainly halted in the G1 phase, and the apoptosis rate and doubling time in this group were significantly higher than those in the blank group and si-CON group. Conclusions: Inhibition of lnc-HOST2 could suppress the proliferation, migration, and invasion and promote the apoptosis of osteosarcoma cells. |
| Databáze: | OpenAIRE |
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