IGF-I, IGF-II, 'free' IGF-I and IGF-binding proteins-2 to -6 during high-dose oestrogen treatment in constitutionally tall girls
| Autor: | Lieve Op De Beeck, Raoul Rooman, Subburaman Mohan, Jaap van Doorn, Marc V. L. Du Caju, Manou Martin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Adolescent medicine.drug_class Endocrinology Diabetes and Metabolism medicine.medical_treatment Insulin-like growth factor-binding protein Endocrinology Insulin-Like Growth Factor II Internal medicine Ethinylestradiol Blood plasma medicine Humans Longitudinal Studies Insulin-Like Growth Factor I Child Chemotherapy biology business.industry Estrogens General Medicine Body Height Insulin-Like Growth Factor Binding Proteins Blot Insulin-Like Growth Factor Binding Protein 2 Insulin-Like Growth Factor Binding Protein 3 Insulin-Like Growth Factor Binding Protein 4 Estrogen Insulin-like growth factor 2 Cohort biology.protein Body Constitution Female Insulin-Like Growth Factor Binding Protein 5 business Insulin-Like Growth Factor Binding Protein 6 hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | European journal of endocrinology |
| ISSN: | 0804-4643 |
| Popis: | OBJECTIVE: To investigate the effect of high-dose oestrogen treatment on IGF-I, IGF-II, free-dissociable IGF-I and the IGF-binding proteins (IGFBP)-2 to -6 in girls with constitutional tall stature. METHODS: In patient cohort 1, blood samples were drawn before and after 3 months of daily oral treatment with 0.1 mg ethinyloestradiol. In cohort 2, samples were collected at the end of the treatment period and 3 to 6 months afterwards. IGFs and IGFBPs were analysed by specific immunoassays and by Western ligand blot. RESULTS: Total IGF-I decreased significantly on oestrogen treatment and increased again after oestrogen withdrawal. Ligand blot analysis showed a clear reduction in a 34 kDa band, corresponding to IGFBP-2, and a strong induction of a 24 kDa band, corresponding to the non-glycosylated form of IGFBP-4. These changes were confirmed by specific immunological methods. The serum levels of IGFBP-3, IGFBP-5 and IGFBP-6 remained unchanged during the first 3 months of treatment. In cohort 2, IGFBP-3 and IGFBP-6 increased after oestrogen withdrawal. Free-dissociable IGF-I fell to 35+/-4% during oestrogen therapy and rose again when the treatment was stopped. CONCLUSIONS: Oestrogens modulate the serum concentrations of several components of the IGF system. The fall in total IGF-I is not explained by a decrease in IGFBPs but probably results from a decreased synthesis. |
| Databáze: | OpenAIRE |
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