A Phelipanche ramosa KAI2 Protein Perceives enzymatically Strigolactones and Isothiocyanates
| Autor: | François-Didier Boyer, Philippe Simier, Jean-Bernard Pouvreau, Vincent Steinmetz, Alexandre de Saint Germain, Philippe Delavault, Guillaume Brun, Sofie Goormachtig, Kris Gevaert, Lukas Braem, Susann Wicke, David Cornu, Anse Jacobs, Vincent Servajean, Emmanuelle Baudu, Guillaume Clavé |
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| Přispěvatelé: | Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
2. Zero hunger 0303 health sciences Obligate [SDV]Life Sciences [q-bio] food and beverages Stimulation Endogeny Biology 01 natural sciences 03 medical and health sciences Phelipanche ramosa Signal perception Biochemistry Germination Weed Receptor 030304 developmental biology 010606 plant biology & botany |
| DOI: | 10.1101/2020.06.09.136473 |
| Popis: | Phelipanche ramosa is an obligate root-parasitic weed threatening major crops in central Europe. For its germination, it has to perceive various structurally diverging host-exuded signals, including isothiocyanates (ITCs) and strigolactones (SLs). However, the receptors involved are still uncharacterized. Here, we identified five putative SL receptors in P. ramosa, of which PrKAI2d3 is involved in seed germination stimulation. We established the high plasticity of PrKAI2d3, allowing interaction with different chemicals, including ITCs. The SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic plants. We provide evidence that the PrKAI2d3 enzymatic activity confers hypersensitivity to SLs. Additionally, we demonstrated that methylbutenolide-OH binds PrKAI2d3 and stimulates P. ramosa germination with a bioactivity comparable to that of ITCs. This study highlights that P. ramosa has extended its signal perception system during evolution, a fact to be considered in the development of specific and efficient biocontrol methods. |
| Databáze: | OpenAIRE |
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