A rotating bed system bioreactor enables cultivation of primary osteoblasts on well-characterized Sponceram regarding structural and flow properties
| Autor: | Thomas Scheper, Martijn van Griensven, Ignacio Ochoa, Stefanie Roeker, Solvig Diederichs, J.A. Sanz-Herrera, Kirstin Suck, Cornelia Kasper, Manuel Doblaré, Fabienne Anton |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Ceramics Materials science Biocompatibility Osteopontin/metabolism Young's modulus Electron Tissue Culture Techniques symbols.namesake Tissue Scaffolds/chemistry Bioreactors Tissue engineering Materials Testing/instrumentation Elastic Modulus Collagen/metabolism Materials Testing Bioreactor Humans Scanning Ceramic Composite material Porosity Microscopy Osteoblasts Tissue Engineering Tissue Scaffolds Reverse Transcriptase Polymerase Chain Reaction Osteoblasts/cytology Biomaterial Tissue Culture Techniques/methods X-Ray Microtomography Biomechanical Phenomena Percolation visual_art symbols visual_art.visual_art_medium Microscopy Electron Scanning Osteopontin Collagen Zirconium Tissue Engineering/instrumentation Biotechnology |
| Zdroj: | Biotechnology progress. 26(3) |
| ISSN: | 1520-6033 |
| Popis: | The development of bone tissue engineering depends on the availability of suitable biomaterials, a well-defined and controlled bioreactor system, and on the use of adequate cells. The biomaterial must fulfill chemical, biological, and mechanical requirements. Besides biocompatibility, the structural and flow characteristics of the biomaterial are of utmost importance for a successful dynamic cultivation of osteoblasts, since fluid percolation within the microstructure must be assured to supply to cells nutrients and waste removal. Therefore, the biomaterial must consist of a three-dimensional structure, exhibit high porosity and present an interconnected porous network. Sponceram, a ZrO(2) based porous ceramic, is characterized in the presented work with regard to its microstructural design. Intrinsic permeability is obtained through a standard Darcy's experiment, while Young's modulus is derived from a two plates stress-strain test in the linear range. Furthermore, the material is applied for the dynamic cultivation of primary osteoblasts in a newly developed rotating bed bioreactor. |
| Databáze: | OpenAIRE |
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