Loading of beclomethasone in liposomes and hyalurosomes improved with mucin as effective approach to counteract the oxidative stress generated by cigarette smoke extract
| Autor: | Maria Manconi, Maria Ferraro, Donatella Valenti, Elisabetta Pace, Maria Letizia Manca, Serena Di Vincenzo, Xavier Fernàndez-Busquets, Catalina Anisoara Peptu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biocompatibility
Estrès oxidatiu General Chemical Engineering pulmonary delivery 02 engineering and technology medicine.disease_cause beclomethasone Article lcsh:Chemistry 03 medical and health sciences mucin Bronchial epithelial cells phospholipid vesicles medicine oxidative stress Mucines General Materials Science 16HBE cells Phospholipids 030304 developmental biology cigarette smoke extract Cigarretes chemistry.chemical_classification 0303 health sciences Reactive oxygen species Liposome Lung Cigarettes Chemistry Vesicle Mucin Mucins 021001 nanoscience & nanotechnology In vitro medicine.anatomical_structure beclomethasone dipropionate lcsh:QD1-999 Oxidative stress Biophysics 0210 nano-technology Fosfolípids |
| Zdroj: | Nanomaterials Nanomaterials, Vol 11, Iss 850, p 850 (2021) Volume 11 Issue 4 Nanomaterials (Basel) (2021). doi:10.3390/nano11040850 info:cnr-pdr/source/autori:Maria Letizia Manca; Maria Ferraro; Elisabetta Pace; Serena Di Vincenzo; Donatella Valenti; Xavier Fernàndez-Busquets; Catalina Anisoara Peptu; Maria Manconi/titolo:Loading of Beclomethasone in Liposomes and Hyalurosomes Improved with Mucin as Effective Approach to Counteract the Oxidative Stress Generated by Cigarette Smoke Extract/doi:10.3390%2Fnano11040850/rivista:Nanomaterials (Basel)/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume Dipòsit Digital de la UB Universidad de Barcelona |
| Popis: | In this work beclomethasone dipropionate was loaded into liposomes and hyalurosomes modified with mucin to improve the ability of the payload to counteract the oxidative stress and involved damages caused by cigarette smoke in the airway. The vesicles were prepared by dispersing all components in the appropriate vehicle and sonicating them, thus avoiding the use of organic solvents. Unilamellar and bilamellar vesicles small in size (~117 nm), homogeneously dispersed (polydispersity index lower than 0.22) and negatively charged (~−11 mV), were obtained. Moreover, these vesicle dispersions were stable for five months at room temperature (~25 °C). In vitro studies performed using the Next Generation Impactor confirmed the suitability of the formulations to be nebulized as they were capable of reaching the last stages of the impactor that mimic the deeper airways, thus improving the deposition of beclomethasone in the target site. Further, biocompatibility studies performed by using 16HBE bronchial epithelial cells confirmed the high biocompatibility and safety of all the vesicles. Among the tested formulations, only mucin-hyalurosomes were capable of effectively counteracting the production of reactive oxygen species (ROS) induced by cigarette smoke extract, suggesting that this formulation may represent a promising tool to reduce the damaging effects of cigarette smoke in the lung tissues, thus reducing the pathogenesis of cigarette smoke-associated diseases such as chronic obstructive pulmonary disease, emphysema, and cancer. |
| Databáze: | OpenAIRE |
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