Análise quantitativa das células das ilhotas pancreáticas em ratos sob efeito de aloxana
| Autor: | Marta Elena Araújo de Abreu, Maria de Fátima Borges, Danielle Patrícia Castanheira Rita, Marcus Aurelho de Lima, Gilberto de Araújo Pereira, Luiz Carlos Reis, Ranaia Santana Tatsukawa, Fabio Castilho Navarro, Lilian Margareth Biagioni de Lima |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
endocrine system
medicine.medical_specialty lcsh:Medicine Ilhotas de Langerhans. Insulina. Glucagon. Somatostatina. Imuno-histoquímica. Aloxana chemistry.chemical_compound Internal medicine Alloxan Male rats medicine geography geography.geographical_feature_category biology business.industry Pancreatic islets lcsh:R General Medicine Islet medicine.anatomical_structure Endocrinology chemistry biology.protein Immunohistochemistry Antibody Pancreas business Quantitative analysis (chemistry) |
| Zdroj: | Medicina, Vol 34, Iss 3/4 (2001) |
| ISSN: | 2176-7262 0076-6046 |
| DOI: | 10.11606/issn.2176-7262.v34i3/4p308-314 |
| Popis: | In the present study, we verified the influence of alloxan over the cells of pancreatic islet of rats, performing quantitative analysis of a , b and d cells through immunohistochemical study. Twenty male rats (Rattus norvegiccus) were used of Wistar line, disposed in 2 groups of 10 rats on each, being a control group and an experimental one. After fasting of 36 hours, 40 ml/Kg of monohydrated of alloxan was administrated by intravenous via and about 30 minutes after, the animal was offered food. At the end of 10 days of observation, animals were sacrificed. Samples of different regions of pancreas were selected and undergone to a histological process and stained with hematoxilin-eosin. To the immunohistochemical study, anti-insulin, anti-glucagon and anti-somatostatin antibodies were used. Quantitative analysis was performed in eight most representative pancreatic islets of each animal. Results were considered significative when p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|