EGFR mutation and HPV infection in sinonasal inverted papilloma and squamous cell carcinoma
| Autor: | José Luis Llorente, Verónica Blanco-Lorenzo, César Álvarez-Marcos, Laura Suárez-Fernández, Virginia N. Cabal, Rocío García-Marín, Blanca Vivanco, Mario Hermsen, Cristina Riobello, Marta Menéndez, Fernando López, Sira Potes-Ares |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Papilloma
Inverted Mutation business.industry Papillomavirus Infections HPV infection General Medicine medicine.disease_cause EGFR Gene Mutation medicine.disease ErbB Receptors 03 medical and health sciences Exon 0302 clinical medicine Otorhinolaryngology Carcinoma Squamous Cell medicine Carcinoma Cancer research Humans Papilloma KRAS 030223 otorhinolaryngology business EGFR inhibitors |
| Zdroj: | Rhinology journal. |
| ISSN: | 0300-0729 |
| Popis: | Background To evaluate the involvement of EGFR signalling and HPV infection in a cohort of inverted sinonasal papilloma (ISP) and sinonasal squamous cell carcinoma (SNSCC) and their value for prognosis and clinical treatment. Methods We analysed 55 ISP, 14 SNSCC associated with ISP (SNSCC-isp) and and 60 SNSCC not associated with ISP (SNSCC-novo) for EGFR gene mutation and copy number gain, protein expression of EGFR and phosporylated EGFR (pEGFR), and HPV-infection and KRAS mutation. Findings were correlated to clinico-pathological and follow-up data. Results We found EGFR exon 20 mutations in 38% (7/18) ISP, in 50% (6/12) SNSCC-isp and in 5% (1/19) SNSCC-novo. EGFR was expressed in 92% of ISP, while pEGFR was observed in 54% (21/39). SNSCC-isp and SNSCC-novo demonstrated comparable expression of EGFR (57% and 33%) and of pEGFR (44% and 38%). We observed an inverse relation between EGFR exon 20 mutation and pEGFR expression. Four of 39 (10%) ISP carried HPV-16. Oncogenic HPV was detected in 3/12 (25%) SNSSC-isp and in 1/8 (13%) SNSCC-novo. KRAS mutations were not detected in any of the samples. HPV infection was inversely correlated with pEGFR expression but not with EGFR mutation. ISP with EGFR activation by mutation or by phosphorylation had longer ISP-free survival, however, neither EGFR exon 20 mutation, pEGFR expression nor HPV infection demonstrated prognostic value in SNSCC. Conclusions EGFR exon 20 mutation is frequent in ISP and SNSCC-isp, while activation of EGFR through phosphorylation also plays an important role. Our data indicate that a large proportion of SNSCC patients could benefit from therapy with modern EGFR inhibitors. |
| Databáze: | OpenAIRE |
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