A Prospective Study to Assess the Optimal Incubation Times for Culture and Aerobic Bacterial Profile in Prosthetic Joint Infections
Autor: | Priya Datta, Varsha Gupta, Mandeep Kaur, Prapti Bora, Pooja Kumari, Ravi Gupta, Jagdish Chander |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Staphylococcus aureus medicine.drug_class Klebsiella pneumoniae Prosthetic joint 030106 microbiology Antibiotics prolonged incubation medicine.disease_cause Incubation period Microbiology 03 medical and health sciences 0302 clinical medicine medicine infections Prospective cohort study Incubation 030222 orthopedics biology business.industry biology.organism_classification Antimicrobial culture Medicine Original Article prosthesis business |
Zdroj: | Journal of Laboratory Physicians Journal of Laboratory Physicians, Vol 13, Iss 03, Pp 224-230 (2021) |
ISSN: | 0974-7826 0974-2727 |
Popis: | Introduction With an increase in the number of total joint arthroplasties, the count of prosthetic joint infections (PJIs) is also increasing and has become a nightmare for an orthopaedic surgeon. Microbiological diagnosis is important for administering definitive antimicrobial treatment. Negative culture reports hamper patient management and prolonged incubation periods have increased the culture yield but at the risk of culture contamination in conventional microbiology settings. Thus, we aimed to optimize the best incubation time for culture and the aerobic bacterial profile of PJIs. Material and Methods Over a year, samples from clinically suspected PJI patients were collected and processed for culture using standard techniques. The samples were incubated for up to 10 days with daily subculturing on to solid media. The bacterial isolates were identified and antibiotic susceptibility was performed. Results Out of 200 patients, 105 were included in the study and samples were collected in triplicate. In 70 cases culture was positive and 35 were culture negative PJIs. Mean incubation days to culture positivity was 3.6 days with 97.14% culture positivity seen by the seventh day of incubation. Twenty-four percent methicillin-resistant Staphylococcus aureus, and more than 50% extended spectrum β lactamase producing Klebsiella pneumoniae and Escherichia coli were isolated. Discussion Culture positivity in PJIs provides definitive evidence of infection and guides the treatment. Increasing the incubation times can help in maximizing the culture yield, and we found that 97.14% pathogens grew within 7 days of incubation. Prolonging it further would not provide an added advantage especially in a resource-constraint setting. |
Databáze: | OpenAIRE |
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