Tumour necrosis factor-α in Barrett's oesophagus: a potential novel mechanism of action

Autor: Christopher C. McConkey, Christopher W. Dawson, Chris Tselepis, S. Jane Darnton, Rob C Stuart, Rebecca Harrison, Nicholas A. Wright, Janusz Jankowski, Robert G. Hardy, Ian Perry
Rok vydání: 2002
Předmět:
Cancer Research
medicine.medical_specialty
Beta-catenin
Esophageal Neoplasms
Transcription
Genetic
medicine.medical_treatment
Adenomatous Polyposis Coli Protein
Blotting
Western
Genes
myc
Adenocarcinoma
Biology
Transfection
Polymerase Chain Reaction
p38 Mitogen-Activated Protein Kinases
Receptors
Tumor Necrosis Factor
Immunoenzyme Techniques
Proto-Oncogene Proteins c-myc
Barrett Esophagus
Antigens
CD
Transcription (biology)
Metaplasia
Internal medicine
Gene expression
Tumor Cells
Cultured
Genetics
medicine
Humans
Enzyme Inhibitors
Molecular Biology
beta Catenin
Oncogene
Tumor Necrosis Factor-alpha
NF-kappa B
Up-Regulation
Cytoskeletal Proteins
Real-time polymerase chain reaction
Endocrinology
Cytokine
Receptors
Tumor Necrosis Factor
Type I
Trans-Activators
Cancer research
biology.protein
Mitogen-Activated Protein Kinases
Signal transduction
medicine.symptom
Signal Transduction
Zdroj: Oncogene. 21:6071-6081
ISSN: 1476-5594
0950-9232
DOI: 10.1038/sj.onc.1205731
Popis: Barrett's metaplasia (BM) is an early lesion in the progression from oesophageal inflammation through dysplasia to the development of Barrett's adenocarcinoma (BA). Previous work indicates that BM and BA are associated with reduced E-cadherin expression and increased cytoplasmic/nuclear pools of its associated protein beta-catenin. beta-catenin participates in Wnt signalling and activates oncogene transcription by complexing with T-cells factors (TCF). One such oncogene is c-myc. We have previously shown that TNF-alpha can down-regulate E-cadherin expression. Here, we assess TNF-alpha expression in Barrett's metaplasia and examine if TNF-alpha can promote beta-catenin mediated transcription of oncogenes in a gastrointestinal model system. Employing immunohistochemistry and Western blot analysis of oesophageal tissue, epithelial expression of TNF-alpha increases with progression along the metaplasia-dysplasia-carcinoma sequence (P
Databáze: OpenAIRE
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