The amount of citrullinated proteins in synovial tissue is related to serum anti-cyclic citrullinated peptide (anti-CCP) antibody levels

Autor: Diego F. Hernández-Ramírez, Elizabeth Olivares-Martínez, Antonio R. Cabral, Luis Llorente, Carlos A. Núñez-Álvarez
Rok vydání: 2015
Předmět:
Male
musculoskeletal diseases
0301 basic medicine
medicine.medical_specialty
Hydrolases
Peptides
Cyclic
Arthritis
Rheumatoid
03 medical and health sciences
Protein-Arginine Deiminase Type 3
0302 clinical medicine
Protein-Arginine Deiminase Type 4
Rheumatology
Western blot
Protein-Arginine Deiminase Type 2
Internal medicine
Osteoarthritis
medicine
Humans
RNA
Messenger
Aged
Autoantibodies
Aged
80 and over
030203 arthritis & rheumatology
Messenger RNA
biology
medicine.diagnostic_test
business.industry
Synovial Membrane
Autoantibody
Anti–citrullinated protein antibody
General Medicine
Middle Aged
medicine.disease
030104 developmental biology
Endocrinology
medicine.anatomical_structure
PADI2
Rheumatoid arthritis
Protein-Arginine Deiminases
biology.protein
Female
Synovial membrane
Antibody
business
Zdroj: Clinical Rheumatology. 35:55-61
ISSN: 1434-9949
0770-3198
Popis: The objective of this study was to determine the relationship between citrullinated proteins in synovial tissue with peripheral anti-citrullinated peptides autoantibodies (ACPA) and peptidylarginine deiminase (PADI) PADI2, PADI3, and PADI4 messenger RNA (mRNA) expressions in synovial tissue and fibroblast-like synoviocytes in rheumatoid arthritis (RA) patients. Eleven RA and 12 osteoarthritis (OA) patients who underwent knee replacement surgery were studied. We detected citrullinated proteins in synovial tissue homogenates by western blot and serum ACPA by ELISA to anti-cyclic citrullinated peptide (anti-CCP) antibodies, and PADI2, PADI3, and PADI4 mRNA expressions in synovial tissue and in fibroblast-like synoviocytes. Patients with high amount of citrullinated proteins in synovial tissue (3 out of 7) have high levels of anti-CCP in serum. However, in the remaining 4 patients, the amount of synovial citrullinated proteins was minimal and their sera showed low levels of anti-CCP antibodies. Furthermore, we observed an increase in PADI2 mRNA expression in RA synovial tissue compared with OA patients (p = 0.02). We detected PADI3 mRNA in the synovial tissue of RA patients, but not in the tissue of OA patients. Even though fibroblast-type synoviocytes in RA are not the main source of PADs in the synovial tissue, they express PADI2 mRNA moderately, PADI4 mRNA weakly, while there is no detectable expression of PADI3 mRNA. In conclusion, we found a variety of citrullinated proteins in the synovial tissue of RA patients and the amount of such proteins is related to serum concentration of anti-CCP antibodies. We identified the presence of PADI3 mRNA expression in synovial tissue and PADI2 and PADI4 mRNA expressions in fibroblast-like synoviocytes from patients with RA.
Databáze: OpenAIRE
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