Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans
| Autor: | Kodiveri Muthukaliannan Gothandam, Ajitha Gomathi, Vitor Vasconcelos, J. G. S. Pavan Kumar |
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| Přispěvatelé: | CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Geologic Sediments Antifungal Agents Metabolite polyketide synthase Pharmaceutical Science phylogeny Andaman and Nicobar Islands chemistry.chemical_compound RNA Ribosomal 16S Drug Discovery Candida albicans antibiotic agent antifungal agent genetics polyketide synthase type I lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) biology drug effect Nucleic acid sequence actinobacteria genetic screening RNA analysis mass fragmentography Corpus albicans Streptomyces unclassified drug Actinobacteria microbial sensitivity test bacterium identification polyketide synthase type II drug isolation RNA 16S 030106 microbiology metabolite India gene sequence Microbial Sensitivity Tests chemistry Article Gas Chromatography-Mass Spectrometry Microbiology non ribosomal peptide synthase 03 medical and health sciences Polyketide synthase controlled study 14. Life underwater drug screening ecosystem mangrove nonhuman bacterium isolate antifungal activity microbiology Actinobacteria extract nucleotide sequence biology.organism_classification 16S ribosomal RNA bacterial strain lcsh:Biology (General) sediment biology.protein drug synthesis Rhizophoraceae nonribosomal peptide synthetase antifungal |
| Zdroj: | Marine Drugs Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Marine Drugs; Volume 16; Issue 2; Pages: 60 Marine Drugs, Vol 16, Iss 2, p 60 (2018) |
| ISSN: | 1660-3397 |
| Popis: | Actinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Andaman and the Nicobar Islands. Identification was carried out using 16S rRNA analysis and biosynthetic genes (Polyketide synthase type I/II and Non-ribosomal peptide synthase) were screened. Actinobacteria were assayed for their antifungal activity against 16 clinical Candida albicans and the compound analysis was performed using gas chromatography-mass spectrometry GC-MS. The 31 actinobacterial strains were isolated and 16S rRNA gene sequencing revealed that this ecosystem is rich on actinobacteria, with Streptomyces as the predominant genus. The PCR based screening of biosynthetic genes revealed the presence of PKS-I in six strains, PKS-II in four strains and NRPS in 11 strains. The isolated actinobacteria VITGAP240 and VITGAP241 (two isolates) were found to have a potential antifungal activity against all the tested C. albicans. GC-MS results revealed that the actinobacterial compounds were belonging to heterocyclic, polyketides and peptides. Overall, the strains possess a wide spectrum of antifungal properties which affords the production of significant bioactive metabolites as potential antibiotics. © 2018 by the authors. Acknowledgments: The authors gratefully acknowledge VIT University, Vellore for the support through Seed Grant for Research. We also thank A. Manikandan, Department of Biotechnology, VIT University for his valuable suggestion in separation of bioactive compounds and their bioactivity predictions of compounds. VV acknowledges FCT Project UID/Multi/04423/2013 and by the Structured Program of R&D&I INNOVMAR—Innovation and Sustainability in the Management and Exploitation of Marine Resources (reference NORTE-01-0145-FEDER-000035, Research Line NOVELMAR), funded by the Northern Regional Operational Program (NORTE2020) through the European Regional Development Fund (ERDF). |
| Databáze: | OpenAIRE |
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