Nicotine exposure impairs germ cell development in human fetal ovaries cultured in vitro
| Autor: | Yi Zhou, Yanni Feng, Lan Li, Shu-Hua Zou, Ze-Li Han, Xiao-Feng Sun, Wei Ge, Yong Zhao, Fan Yang, Massimo De Felici, Xun-Si Qin, Wei Shen, Shun-Feng Cheng |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Aging germ cells Nicotine Apoptosis DNA damage Germ cells Human fetal ovary Dose-Response Relationship Drug Female Fetus Germ Cells Humans Nicotinic Agonists Ovary Tissue Culture Techniques Somatic cell Biology Dose-Response Relationship Andrology 03 medical and health sciences 0302 clinical medicine medicine Ovarian reserve Settore BIO/17 030219 obstetrics & reproductive medicine apoptosis Cell Biology human fetal ovary medicine.disease Menopause Nicotinic acetylcholine receptor 030104 developmental biology medicine.anatomical_structure Drug Germ cell medicine.drug Research Paper |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| Popis: | In the present paper, we found that human fetal ovaries (at ~16 weeks) express the transcripts for several subunits of the nicotinic acetylcholine receptor (nAChR). Exposure to the drug in vitro resulted in the marked increase of apoptosis in the ovaries in a time and dose-dependent manner. Evidence that adverse nicotine effects are potentially due to an increased level of reactive oxygen species (ROS) and consequent DNA damage, both in the ovarian somatic cells and germ cells, are reported. After 4 days of culture, exposure to 1 mM and 10 mM nicotine caused a 50% and 75% decrease, respectively, in the number of oogonia/oocytes present in the fetal ovaries. These results represent the first indication that nicotine may directly cause apoptosis in cells of the fetal human ovary and may lead to a reduction of the ovarian reserve oocytes and consequent precocious menopause in mothers smoking during pregnancy. |
| Databáze: | OpenAIRE |
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