Biphasic mechanosensitivity of TCR mediated adhesion of T lymphocytes
| Autor: | Astrid Wahl, Pierre-Henri Puech, Kheya Sengupta, Laurent Limozin, Céline Dinet, Pierre Dillard |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
musculoskeletal diseases
0303 health sciences animal structures biology Chemistry T-cell receptor Integrin Stiffness Adhesion macromolecular substances equipment and supplies 03 medical and health sciences Immune recognition 0302 clinical medicine medicine biology.protein Biophysics Mechanosensitive channels medicine.symptom Antigen-presenting cell 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/232041 |
| Popis: | Force sensitivity of the T cell receptor (TCR) is now believed to be essential for immune recognition, but cellular mechanosensitivity of T cells is still poorly understood. Here we show that T cells adhering via the TCR-complex respond to environmental stiffness in an unusual biphasic fashion. As the stiffness increases, adhesion and spreading first increase, then decrease, attaining their maximal values on an optimally stiff surface, with stiffness comparable to certain antigen presenting cells. Remarkably, in presence of additional ligands for the integrin LFA-1, spreading increases monotonously with stiffness up to a saturation value. Using a mesoscopic semi-analytical model linking spreading to molecular characteristics of bonds, we identify force sensitivity of the off-rate and the effective bond stiffness as the crucial parameters that determine monotonic or biphasic mechanosensitive behavior. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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