Next-Generation Sequencing of Local Romanian Tomato Varieties and Bioinformatics Analysis of the
| Autor: | Anca-Amalia Udriște, Mihaela Iordachescu, Roxana Ciceoi, Liliana Bădulescu |
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| Rok vydání: | 2022 |
| Předmět: |
Romania
Organic Chemistry Computational Biology High-Throughput Nucleotide Sequencing General Medicine Verticillium Catalysis Computer Science Applications Inorganic Chemistry Plant Breeding Solanum lycopersicum Gene Expression Regulation Plant Physical and Theoretical Chemistry Molecular Biology Spectroscopy Solanum lycopersicum L NGS genetic variability biotic stress Romanian tomato Verticillium wilt Plant Diseases Plant Proteins |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 17; Pages: 9750 |
| ISSN: | 1422-0067 |
| Popis: | Genetic variability is extremely important, not only for the species’ adaptation to environmental challenges, but also for the creation of novel varieties through plant breeding. Tomato is an important vegetable crop, as well as a model species in numerous genomic studies. Its genome was fully sequenced in 2012 for the ‘Heinz 1706’ variety, and since then, resequencing efforts have revealed genetic variability data that can be used for multiple purposes, including triggering mechanisms of biotic and abiotic stress resistance. The present study focused on the analysis of the genome variation for eight Romanian local tomato varieties using next-generation sequencing technique, and as a case study, the sequence analysis of the Ve1 and Ve2 loci, to determine which genotypes might be good candidates for future breeding of tomato varieties resistant to Verticillium species. The analysis of the Ve locus identified several genotypes that could be donors of the Ve1 gene conferring resistance to Verticillium race 1. Sequencing for the first time Romanian genotypes enriched the existing data on various world tomato genetic resources, but also opened the way for the molecular breeding in Romania. Plant breeders can use these data to create novel tomato varieties adapted to the ever-changing environment. |
| Databáze: | OpenAIRE |
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