Silencing of long non-coding RNA DLX6-AS1 weakens neuroblastoma progression by the miR-513c-5p/PLK4 axis
| Autor: | Peisheng Jia, Tingting Wu, Hui-Qiong Liu, Huai-Li Wang, Erhu Wei |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Cell cycle checkpoint Clinical Biochemistry Cell Mice Nude Apoptosis Protein Serine-Threonine Kinases Biochemistry 03 medical and health sciences Mice Neuroblastoma 0302 clinical medicine Downregulation and upregulation Genetics medicine Biomarkers Tumor Tumor Cells Cultured Gene silencing Animals Humans RNA Antisense Viability assay Molecular Biology Cell Proliferation Homeodomain Proteins Mice Inbred BALB C Chemistry DLX6 antisense RNA 1 Cell Biology medicine.disease Prognosis Xenograft Model Antitumor Assays Long non-coding RNA Gene Expression Regulation Neoplastic Survival Rate MicroRNAs 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research RNA Long Noncoding |
| Zdroj: | IUBMB lifeREFERENCES. 72(12) |
| ISSN: | 1521-6551 |
| Popis: | Emerging evidence has demonstrated the crucial roles of long noncoding RNAs in human cancers, including neuroblastoma (NB). DLX6 antisense RNA 1 (DLX6-AS1) has been identified as an oncogenic driver in NB. However, the mechanisms of DLX6-AS1 in NB progression are not fully understood. Our data showed that DLX6-AS1 was significantly overexpressed in NB tissues and cells. Moreover, DLX6-AS1 silencing repressed NB cell viability, colony formation, migration, and invasion, and promoted cell cycle arrest and apoptosis in vitro, as well as decreased tumor growth in vivo. Mechanistically, DLX6-AS1 operated as a miR-513c-5p sponge. MiR-513c-5p mediated the regulation of DLX6-AS1 on NB cell malignant progression in vitro. PLK4 was a target of miR-513c-5p- and DLX6-AS1-controlled PLK4 expression via sponging miR-513c-5p. Furthermore, the suppressive effect of miR-513c-5p overexpression on NB cell malignant progression in vitro was reversed by PLK4 upregulation. Our findings identified a novel regulatory mechanism, the DLX6-AS1/miR-513c-5p/PLK4 axis, in NB progression, highlighting a strong rationale for developing DLX6-AS1 as a new target for NB management. |
| Databáze: | OpenAIRE |
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