Expression of functional trk protooncogene in human monocytes
| Autor: | Uwe Otten, Patricia B. Ehrhard, Joachim Bauer, Ursula Ganter, A Stalder |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
animal structures medicine.medical_treatment Molecular Sequence Data Gene Expression Receptors Nerve Growth Factor Biology Polymerase Chain Reaction Monocytes Proto-Oncogene Proteins Internal medicine Proto-Oncogenes Tumor Cells Cultured medicine Humans Nerve Growth Factors RNA Messenger Receptor trkA Cells Cultured Multidisciplinary Base Sequence U937 cell Growth factor Monocyte Cell Differentiation Protein-Tyrosine Kinases Respiratory burst Cell biology Kinetics Nerve growth factor Endocrinology medicine.anatomical_structure Cytokine Bucladesine Oligodeoxyribonucleotides nervous system Trk receptor embryonic structures biology.protein Tetradecanoylphorbol Acetate Signal Transduction Research Article Neurotrophin |
| Zdroj: | Proceedings of the National Academy of Sciences. 90:5423-5427 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.90.12.5423 |
| Popis: | There is increasing evidence that neurotrophins, including nerve growth factor (NGF), exert specific effects on cells of the immune system in addition to their neurotrophic actions. This report shows that human monocytes express the trk protooncogene, encoding the signal-transducing receptor unit for NGF. This receptor is functional, since interaction of NGF with monocytes triggered a respiratory burst, the major component of monocyte cytotoxic activity. During in vitro differentiation of human blood monocytes to macrophages trk expression decreased, suggesting a maturation-dependent trk expression decreased, suggesting a maturation-dependent trk regulation. Treatment of monocytes with Staphylococcus aureus Cowan I, a potent activator of monocytes, stimulated trk mRNA synthesis in a time-dependent way, implying a modulatory role for NGF in immune functions. The finding that dibutyryl cAMP elicited a time-dependent trk induction in monocytes as well as in phorbol ester-differentiated promonocytic U937 cells indicates that adenylate cyclase is involved in monocytic trk regulation. These results suggest that NGF, in addition to its neurotrophic function, is an immunoregulatory cytokine acting on monocytes. |
| Databáze: | OpenAIRE |
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