Astrogliosis and decreased neural viability as consequences of early consumption of aspartame and acesulfame potassium in male Wistar rats
| Autor: | María Lilia López-Narváez, Carlos Alfonso Tovilla-Zárate, Alma Delia Genis-Mendoza, Juan C. Díaz-Zagoya, Valeria Quintanar-Jurado, Isela Esther Juárez-Rojop, Thelma Beatriz González-Castro, Anayelly Solis-Medina, Humberto Nicolini, Ileana Gallegos-Silva, Yazmín Hernández-Díaz, José Jaime Martínez-Magaña |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Cell Survival Acesulfame potassium Thiazines Hippocampus Biochemistry Amygdala 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Gliosis Rats Wistar Prefrontal cortex Aspartame Neurons Glial fibrillary acidic protein biology Chemistry Brain medicine.disease Astrogliosis Rats 030104 developmental biology Endocrinology medicine.anatomical_structure nervous system Hypothalamus Sweetening Agents biology.protein Neurology (clinical) 030217 neurology & neurosurgery |
| Zdroj: | Metabolic brain disease. 33(6) |
| ISSN: | 1573-7365 |
| Popis: | Artificial sweeteners are mainly used as substitutes for sucrose derivates. In this study, we analyzed if the chronic consumption of aspartame or acesulfame potassium at an early age, produces histological alterations, astrogliosis and decreased neuronal viability, in hippocampus, prefrontal cortex, amygdala and hypothalamus of male Wistar rats. A histological analysis was performed on male Wistar rats that consumed aspartame or acesulfame potassium during 90 days, initiating the consumption of sweeteners immediately after weaning. The evaluation of neuronal morphology in different areas of the brain was performed with hematoxylin - eosin staining. To measure astrogliosis and neuronal viability, we used the immunohistochemical technique, with the glial fibrillary acidic protein immunomodulators (GFAP) and with neuronal-specific enolase (NSE). The consumption of aspartame or acesulfame potassium promoted morphological changes of neurons including increased pyknotic nuclei and vacuolization in all the brain areas studied. In hippocampus, prefrontal cortex, amygdala and hypothalamus, astrogliosis and reduction of neural viability were observed in sweeteners consumers in comparison with the control group. Chronic consumption of ASP and ACK from early stages of development and during long periods, may promote neural modifications, astrogliosis and decrease neuronal viability in prefrontal cortex, amygdala, hippocampus, and hypothalamus. |
| Databáze: | OpenAIRE |
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