Circadian Control of Transcriptional and Metabolic Rhythms in Primary Hepatocytes
| Autor: | Sung Kook Chun, Selma Masri |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Clock-controlled gene expression
Liver Disease Article Circadian Rhythm Synchronization of the circadian clock Luciferase reporter assays Gene Expression Regulation Glucose production assays Circadian Clocks Luminescent Measurements Primary hepatocyte cell culture Hepatocytes Primary hepatocyte isolation Biochemistry and Cell Biology Luciferases Digestive Diseases Sleep Research Other Chemical Sciences Metabolic and endocrine Developmental Biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071622483 Methods Mol Biol |
| DOI: | 10.1007/978-1-0716-2249-0_11 |
| Popis: | Isolation of primary hepatocytes and culturing these cells ex vivo provides a powerful platform to model liver physiology in vivo. Primary hepatocytes can be cultured for several days, the circadian clock can be synchronized, and these primary cells can be utilized for functional gene regulation analysis and metabolic studies. In this chapter, we describe detailed methodology for isolation of viable primary hepatocytes, techniques for culturing these cells, methods for synchronization of the circadian clock, transfection and luciferase reporter analysis, as well as glucose production assays as a functional readout of metabolic state. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|