Proteomic analysis of nasal mucus samples of healthy patients and patients with chronic rhinosinusitis
| Autor: | Tim Chataway, Sarah Vreugde, Peter-John Wormald, Alkis J. Psaltis, Stephen Shih-Teng Kao, Alex D. Colella, Mahnaz Ramezanpour, Ahmed Bassiouni, John W. Finnie, Nusha Chegeni |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male Proteomics Proteome Chronic rhinosinusitis Immunology Pathogenesis 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Nasal mucus otorhinolaryngologic diseases Immunology and Allergy Medicine Humans Nasal polyps Sinusitis 030223 otorhinolaryngology Aged Rhinitis business.industry respiratory system Middle Aged medicine.disease Mucus Nasal Mucosa 030104 developmental biology Chronic Disease Female business |
| Zdroj: | The Journal of allergy and clinical immunology. 147(1) |
| ISSN: | 1097-6825 |
| Popis: | Chronic rhinosinusitis (CRS) has a complex and multifactorial pathogenesis with a heterogeneous inflammatory profile. Proteomic analysis of nasal mucus may enable further understanding of protein abundances and biologic processes present in CRS and its endotypes compared with in healthy patients.Our aim was to determine differences in the nasal mucus proteome of healthy patients and patients with CRS.Nasal mucus was obtained from healthy patients, patients with CRS without nasal polyps (CRSsNP), and patients with CRS with nasal polyps (CRSwNP) before surgery. Gel electrophoresis was performed to fractionate the complex protein extracts before mass spectrometry analysis. Gene set enrichment analysis was performed on differentially expressed proteins.A total of 33 patients were included in this study (12 healthy, 10 with CRSsNP, and 11 with CRSwNP). In all, 1142 proteins were identified in mucus samples from healthy patients, 761 in mucus samples from patients with CRSsNP, and 998 in mucus samples from patients with CRSwNP. Dysfunction in immunologic pathways, reduced cellular signaling, and increased cellular metabolism with associated tissue remodeling pathways were present in patients with CRS compared with in healthy patients.Significant downregulation of mucosal immunity and antioxidant pathways with increased tissue modeling processes may account for the clinical manifestations of CRS. Ultimately, the differing proteome and biologic processes provide further insight into CRS pathogenesis and its endotypes. |
| Databáze: | OpenAIRE |
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