A novel method for quantifying axon degeneration
| Autor: | Bruno Carturan, Philip A. Barker, Julien Gibon, Robin M. Hallett, Andrés de Léon, Aaron D. Johnstone |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Embryology Cell lcsh:Medicine Fluorescent Antibody Technique Immunostaining Degeneration (medical) Biochemistry Physical Chemistry Nerve Fibers 0302 clinical medicine Tubulin Animal Cells Ganglia Spinal Nerve Growth Factor Medicine and Health Sciences lcsh:Science Cytoskeleton Egtazic Acid Cells Cultured Neurons Staining Neuronal Death Multidisciplinary Cell Death Chelation Neurodegenerative Diseases Immunohistochemistry Molecular Imaging Ganglion Chemistry medicine.anatomical_structure Neurology Cell Processes Physical Sciences Cellular Types Anatomy Research Article Biology Research and Analysis Methods Neuroprotection 03 medical and health sciences Tubulins Extracellular medicine Calcium Chelating Agents Chemical Bonding lcsh:R Embryos Biology and Life Sciences Proteins Cell Biology Axons Cytoskeletal Proteins Biological Tissue 030104 developmental biology Nerve growth factor nervous system Specimen Preparation and Treatment Cellular Neuroscience lcsh:Q Calcium Ganglia Neuroscience Biomarkers 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 7, p e0199570 (2018) |
| ISSN: | 1932-6203 |
| Popis: | Axons normally degenerate during development of the mammalian nervous system, but dysregulation of the same genetically-encoded destructive cellular machinery can destroy crucial structures during adult neurodegenerative diseases. Nerve growth factor (NGF) withdrawal from dorsal root ganglia (DRG) axons is a well-established in vitro experimental model for biochemical and cell biological studies of developmental degeneration. Definitive methods for measuring axon degeneration have been lacking and here we report a novel method of axon degeneration quantification from bulk cultures of DRG that enables objective and automated measurement of axonal density over the entire field of radial axon outgrowth from the ganglion. As proof of principal, this new method, written as an R script called Axoquant 2.0, was used to examine the role of extracellular Ca2+ in the execution of cytoskeletal disassembly during degeneration of NGF-deprived DRG axons. This method can be easily applied to examine degenerative or neuroprotective effects of gene manipulations and pharmacological interventions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|