Antimony-induced alterations in thiol homeostasis and adenine nucleotide status in cultured cardiac myocytes
| Autor: | M.A. Tirmenstein, Mark Toraason, H.E. Wey, John E. Snawder, Patty I. Mathias |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
animal structures Cell Survival animal diseases Pyruvate Dehydrogenase Complex Biology Toxicology medicine.disease_cause Rats Sprague-Dawley Schistosomicides chemistry.chemical_compound fluids and secretions Adenine nucleotide Internal medicine medicine Animals Myocyte Cells Cultured chemistry.chemical_classification Glutathione Disulfide L-Lactate Dehydrogenase Adenine Nucleotides Myocardium Glutathione peroxidase Heart Antimony Potassium Tartrate Glutathione Pyruvate dehydrogenase complex Rats body regions Oxidative Stress Endocrinology chemistry Toxicity cardiovascular system Glutathione disulfide Oxidoreductases Oxidative stress |
| Zdroj: | Toxicology. 119:203-211 |
| ISSN: | 0300-483X |
| DOI: | 10.1016/s0300-483x(97)03628-7 |
| Popis: | Cultured cardiac myocytes were exposed for up to 4 h to 50 and 100 microM potassium antimonyl tartrate (PAT). After 4 h, 50 and 100 microM PAT killed 14 and 33% respectively of the cardiac myocytes. PAT-induced alterations in both protein and nonprotein thiol homeostasis. Transient increases in oxidized glutathione disulfide (GSSG) levels were detected after cells were treated with 100 microM PAT for 2 h. After 4 h, both concentrations of PAT significantly depleted reduced glutathione (GSH) levels. Protein thiols levels were also decreased after a 2-h exposure to 50 and 100 microM PAT. Cells treated with 50 microM and 100 microM PAT had a 15% and 40% reduction respectively in protein thiols after 4 h. PAT also significantly inhibited glutathione peroxidase and pyruvate dehydrogenase activity in cardiac myocytes. Pyruvate dehydrogenase activity levels were inhibited as early as 1 h after cells were treated with both concentrations of PAT. Cardiac myocyte ATP levels were also decreased by PAT, but only after a 4-h exposure to 50 microM and 100 microM PAT. Decreases in cellular ATP levels paralleled PAT toxicity put appeared to be secondary to other cellular changes initiated by PAT exposure. |
| Databáze: | OpenAIRE |
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