Atypical cadherin FAT4 orchestrates lymphatic endothelial cell polarity in response to flow
| Autor: | Genevieve A. Secker, Kelly L. Betterman, Helen McNeill, Benjamin M. Hogan, Lydia Sorokin, Drew L. Sutton, Lillian Lim, Naoyuki Miura, Mark L. Kahn, Natasha L. Harvey, Anna Oszmiana, Jan Kazenwadel |
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| Přispěvatelé: | Betterman, Kelly L, Sutton, Drew L, Secker, Genevieve A, Kazenwadel, Jan, Oszmiana, Anna, Lim, Lillian, Miura, Naoyuki, Sorokin, Lydia, Hogan, Benjamin M, Kahn, Mark L, McNeill, Helen, Harvey, Natasha L |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Mice Transgenic Biology 03 medical and health sciences Mice 0302 clinical medicine Cell polarity FAT4 medicine Lymphatic vessel Animals Humans Lymphedema Lymphangiogenesis genes Lymphatic Vessels Hippo signaling pathway Cadherin Cell Polarity Endothelial Cells General Medicine Syndrome medicine.disease Cadherins human lymphedema syndrome Cell biology Endothelial stem cell GATA2 Transcription Factor Hennekam syndrome 030104 developmental biology Lymphatic system medicine.anatomical_structure 030220 oncology & carcinogenesis Female calcium binding EGF domains 1 (CCBE1) Research Article |
| Zdroj: | J Clin Invest |
| Popis: | The atypical cadherin FAT4 has established roles in the regulation of planar cell polarity and Hippo pathway signaling that are cell context dependent. The recent identification of FAT4 mutations in Hennekam syndrome, features of which include lymphedema, lymphangiectasia, and mental retardation, uncovered an important role for FAT4 in the lymphatic vasculature. Hennekam syndrome is also caused by mutations in collagen and calcium binding EGF domains 1 (CCBE1)and ADAM metallopeptidase with thrombospondin type 1 motif 3 (ADAMTS3), encoding a matrix protein and protease,respectively, that regulate activity of the key prolymphangiogenic VEGF-C/VEGFR3 signaling axis by facilitating the proteolytic cleavage and activation of VEGF-C. The fact that FAT4, CCBE1, and ADAMTS3 mutations underlie Hennekam syndrome suggested that all 3 genes might function in a common pathway. We identified FAT4 as a target gene of GATA binding protein 2 (GATA2), a key transcriptional regulator of lymphatic vascular development and, in particular, lymphatic vessel valve development. Here, we demonstrate that FAT4 functions in a lymphatic endothelial cell–autonomous manner to control cell polarity in response to flow and is required for lymphatic vessel morphogenesis throughout development.Our data reveal a crucial role for FAT4 in lymphangiogenesis and shed light on the mechanistic basis by which FAT4mutations underlie a human lymphedema syndrome usc Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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