Analysis of the effects of dietary fat on body and skin lipids of hamsters by Raman spectroscopy
| Autor: | Yui Maeda, Phiranuphon Meksiarun, Tatsuya Hiroi, Bibin Bintang Andriana, Hidetoshi Sato |
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| Rok vydání: | 2015 |
| Předmět: |
Lipid composition
Adipose tissue Conjugated system Spectrum Analysis Raman Biochemistry Analytical Chemistry symbols.namesake chemistry.chemical_compound Cricetinae Electrochemistry Animals Environmental Chemistry Spectroscopy Dietary fat Skin Triglyceride Chemistry Lipid metabolism Lipid Metabolism Dietary Fats Dietary fat intake symbols Feasibility Studies Lipid Peroxidation Raman spectroscopy |
| Zdroj: | The Analyst. 140:4238-4244 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c5an00076a |
| Popis: | Raman spectroscopy has previously been applied for studying lipid metabolism. In this study, a ball lens-installed hollow optical fiber Raman probe (BHRP) was used for the noninvasive measurement of skin lipids in hamsters. Our analysis suggested that multi-unsaturated lipids, once converted into a structure containing conjugated double bonds, were oxidized to form peroxides. These results were applied for analyzing lipid metabolism in adipose and skin tissues in hamsters fed tricaprin, saturated medium-chain triglyceride and trilinolein, unsaturated long-chain triglyceride fat diets. Unsaturated lipids formed conjugated structures in skin tissue but not in adipose tissue. Principal component analysis (PCA) revealed that the dietary fat intake correlated strongly with lipid composition in body and skin tissues. Hence, the present results successfully demonstrate that Raman spectroscopy with a BHRP can be a powerful tool for analyzing lipid metabolism. |
| Databáze: | OpenAIRE |
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