Development and validation of a hydrophilic interaction liquid chromatography with tandem mass spectrometry method for the simultaneous detection and quantification of etilefrine and oxilofrine in equine blood plasma and urine
| Autor: | Devan Loganathan, Rong Yi, Sarah Zhao, Noel Kong, Barbara Morrissey, Geoffrey Lam, Jasmeet Sandhu |
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| Rok vydání: | 2014 |
| Předmět: |
Detection limit
Doping in Sports Ephedrine Analyte Chromatography Cardiotonic Agents Chemistry Hydrophilic interaction chromatography Filtration and Separation Urine Tandem mass spectrometry Oxilofrine Analytical Chemistry Etilefrine Tandem Mass Spectrometry medicine Animals Sample preparation Horses Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of separation science. 37(21) |
| ISSN: | 1615-9314 |
| Popis: | A sensitive hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method was developed and validated for the simultaneous detection and quantification of etilefrine and oxilofrine in equine blood plasma and urine. The method is highly sensitive and specific with good precision and accuracy. In plasma the limit of detection and limit of quantification are 0.03 and 0.1 ng/mL, respectively, for both analytes. In urine the limit of detection and limit of quantification are 0.3 and 1 ng/mL, respectively, for both analytes. The suitability of the method for doping control analysis in equine species is demonstrated by analyzing postadministration samples collected after a single intravenous administration of 50 mg etilefrine to a standardbred mare. Etilefrine was detected up to 120 h in urine and up to 48 h in plasma. Etilefrine is highly conjugated in equine urine whereas it exists in the free form in equine plasma. Therefore, enzyme hydrolysis prior to sample preparation is recommended for the detection and quantification of etilefrine and oxilofrine in equine urine. |
| Databáze: | OpenAIRE |
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