The ABC Transporter ABCG1 Is Required for Suberin Formation in Potato Tuber Periderm
| Autor: | Sabine Rosahl, Ramona Landgraf, Nadezhda Frolova, Gerd Hause, Christoph Böttcher, Sophia Sonnewald, Simone Altmann, Ulrike Smolka, Lennart Eschen-Lippold, Dierk Scheel, Nadine Strehmel, Melanie Senning, Benjamin Weigel |
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| Rok vydání: | 2014 |
| Předmět: |
Transgene
ATP-binding cassette transporter Plant Science Biology Gene Expression Regulation Plant RNA interference Suberin Exodermis Botany Gene Research Articles Oligonucleotide Array Sequence Analysis Plant Proteins Solanum tuberosum Regulation of gene expression Cell Membrane fungi food and beverages Biological Transport Cell Biology Plants Genetically Modified Lipids Biochemistry ATP-Binding Cassette Transporters RNA Interference lipids (amino acids peptides and proteins) |
| Zdroj: | The Plant Cell. 26:3403-3415 |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.114.124776 |
| Popis: | The lipid biopolymer suberin plays a major role as a barrier both at plant-environment interfaces and in internal tissues, restricting water and nutrient transport. In potato (Solanum tuberosum), tuber integrity is dependent on suberized periderm. Using microarray analyses, we identified ABCG1, encoding an ABC transporter, as a gene responsive to the pathogen-associated molecular pattern Pep-13. Further analyses revealed that ABCG1 is expressed in roots and tuber periderm, as well as in wounded leaves. Transgenic ABCG1-RNAi potato plants with downregulated expression of ABCG1 display major alterations in both root and tuber morphology, whereas the aerial part of the ABCG1-RNAi plants appear normal. The tuber periderm and root exodermis show reduced suberin staining and disorganized cell layers. Metabolite analyses revealed reduction of esterified suberin components and hyperaccumulation of putative suberin precursors in the tuber periderm of RNA interference plants, suggesting that ABCG1 is required for the export of suberin components. |
| Databáze: | OpenAIRE |
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