Mononuclear phagocytic cells in human milk: HLA-DR and Fc gamma R ligand expression
| Autor: | Richard A. Rivas, Ildy M. Katona, Ayman E. El-Mohandes |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
medicine.medical_specialty Neutrophils Cytological Techniques Integrin alphaXbeta2 Biology Interferon-gamma Immune system Antigen Internal medicine medicine HLA-DR Macrophage Humans Interferon gamma Receptor HLA-DR Antigen Milk Human Staining and Labeling Monocyte Macrophages Receptors IgG HLA-DR Antigens Molecular biology medicine.anatomical_structure Endocrinology Spectrometry Fluorescence Pediatrics Perinatology and Child Health Female Developmental Biology medicine.drug |
| Zdroj: | Biology of the neonate. 66(4) |
| ISSN: | 0006-3126 |
| Popis: | The study of the cellular immune components of human milk is essential in the understanding of the role human milk may play in protecting the nursing infant against infection. We have investigated some phenotypic characteristics of breast milk macrophages (BMM) and have compared them to the characteristics of adult peripheral blood monocytes (PBM) by using dual parameter flow microfluorometry. Most BMM expressed the monocyte/macrophage markers Leu-M3 and Leu-M5. The latter marker was present in high density (bright) on BMM, but the density of expression of Leu-M3 was higher on PBM than on BMM [median fluorescence intensity (MFI) 409 ± 105 versus 203 ± 106, p = 0.02]. The percentage of BMM (98 ± 2) that expressed the HLA-DR antigen did not differ significantly from PBM, but the density of expression was higher on BMM (MFI 318 ± 56 versus 264 ± 41, p = 0.03). The HLA-DR expression of BMM was further enhanced after incubation with interferon-γ for 36 h; however, receptor for interleukin-2 could not be induced on BMM by this treatment. The expression of the three classes of FcγR was lower on BMM than on PBM, in percentage (FcγRI 56 ± 23 versus 79 ± 17%, p = 0.02), density of expression (FcγRIII MFI 71 ± 20 versus 153 ± 73, p = 0.002), or both (FcγRII 74 ± 22% versus 94 ± 12%, p = 0.02, and MFI 115 ± 53 versus 202 ± 59, p = 0.003). These findings help to define the BMM as a Leu-M3+ (dull), HLA-DR ± (bright), phenotypically mature macrophage with a specifically low expression of the FcγR ligands. These cell surface characteristics could be ontogenic in nature, or could reflect pregnancy-induced down-regulation of the maternal immune system. |
| Databáze: | OpenAIRE |
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