Expression polymorphism at the ARPC 4 locus links the actin cytoskeleton with quantitative disease resistance to Sclerotinia sclerotiorum in Arabidopsis thaliana

Autor: Rémy Vincent, Dominique Roby, Sylvain Raffaele, Derry Voisin, Pierre Sadon, Aurélie Le Ru, Thomas Badet, Ophélie Léger, Marielle Barascud, Claudine Balagué
Přispěvatelé: Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Plateforme TRI FR-AIB, Fédération de Recherche Agrobiosciences, Interactions et Biodiversité (FR AIB), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Toulouse Réseau Imagerie-Genotoul ( TRI-Genotoul), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Agence Nationale de la Recherche : ANR-10-KBBE-0005, French Laboratory of Excellence project TULIP : ANR-10-LABX-41 ANR-11-IDEX-0002-02, European Project: 336808,EC:FP7:ERC,ERC-2013-StG,VARIWHIM(2013), Plateforme Imagerie Microscopie, Fédération de Recherche FR3450, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
actin cytoskeleton
expression polymorphism
Arabidopsis thaliana
Physiology
genome-wide association mapping
Arabidopsis
Locus (genetics)
Plant Science
Biology
Plant disease resistance
Cell morphology
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Ascomycota
Gene Expression Regulation
Plant
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
fungal pathogen
Gene
Alleles
ComputingMilieux_MISCELLANEOUS
Disease Resistance
Plant Diseases
Ecotype
Genetics
Polymorphism
Genetic
Callose
Sclerotinia sclerotiorum
Haplotype
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
biology.organism_classification
Actin cytoskeleton
030104 developmental biology
chemistry
quantitative disease resistance
Genetic Loci
Mutation
Genome-Wide Association Study
010606 plant biology & botany
callose
Zdroj: New Phytologist
New Phytologist, 2019, 222 (1), pp.480-496. ⟨10.1111/nph.15580⟩
New Phytologist, Wiley, 2019, 222 (1), pp.480-496. ⟨10.1111/nph.15580⟩
ISSN: 0028-646X
1469-8137
Popis: International audience; Quantitative disease resistance (QDR) is a form of plant immunity widespread in nature, and the only one active against broad host range fungal pathogens. The genetic determinants of QDR are complex and largely unknown, and are thought to rely partly on genes controlling plant morphology and development. We used genome-wide association mapping in Arabidopsis thaliana to identify ARPC4 as associated with QDR against the necrotrophic fungal pathogen Sclerotinia sclerotiorum. Mutants impaired in ARPC4 showed enhanced susceptibility to S. sclerotiorum, defects in the structure of the actin filaments and in their responsiveness to S. sclerotiorum. Disruption of ARPC4 also alters callose deposition and the expression of defense-related genes upon S. sclerotiorum infection. Analysis of ARPC4 diversity in A. thaliana identified one haplotype (ARPC4(R)) showing a c. 1 kbp insertion in ARPC4 regulatory region and associated with higher level of QDR. Accessions from the ARPC4(R) haplotype showed enhanced ARPC4 expression upon S. sclerotiorum challenge, indicating that polymorphisms in ARPC4 regulatory region are associated with enhanced QDR. This work identifies a novel actor of plant QDR against a fungal pathogen and provides a prime example of genetic mechanisms leading to the recruitment of cell morphology processes in plant immunity.
Databáze: OpenAIRE
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