Chronic Exercise Reduces CETP and Mesterolone Treatment Counteracts Exercise Benefits on Plasma Lipoproteins Profile: Studies in Transgenic Mice
| Autor: | Laura Lauand Sampaio Teixeira, J.A. Berti, A.C. Casquero, Helena C. F. Oliveira |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetically modified mouse medicine.medical_specialty Very low-density lipoprotein Clinical chemistry Lipoproteins Down-Regulation Mice Transgenic 030204 cardiovascular system & hematology Biochemistry 03 medical and health sciences Mice 0302 clinical medicine Anabolic Agents Internal medicine Cholesterylester transfer protein Mesterolone Medicine Animals Humans Testosterone Swimming biology business.industry Organic Chemistry Cell Biology Cholesterol Ester Transfer Proteins carbohydrates (lipids) 030104 developmental biology Endocrinology Receptors LDL Models Animal biology.protein lipids (amino acids peptides and proteins) Sedentary Behavior business Lipoprotein Lipidology medicine.drug |
| Zdroj: | Lipids. 52(12) |
| ISSN: | 1558-9307 |
| Popis: | Regular exercise and anabolic androgenic steroids have opposing effects on the plasma lipoprotein profile and risk of cardio-metabolic diseases in humans. Studies in humans and animal models show conflicting results. Here, we used a mice model genetically modified to mimic human lipoprotein profile and metabolism. They under-express the endogenous LDL receptor gene (R1) and express a human transgene encoding the cholesteryl ester transfer protein (CETP), normally absent in mice. The present study was designed to evaluate the independent and interactive effects of testosterone supplementation, exercise training and CETP expression on the plasma lipoprotein profile and CETP activity. CETP/R1 and R1 mice were submitted to a 6-week swimming training and mesterolone (MEST) supplementation in the last 3 weeks. MEST treatment increased markedly LDL levels (40%) in sedentary CETP/R1 mice and reduced HDL levels in exercised R1 mice (18%). A multifactorial ANOVA revealed the independent effects of each factor, as follows. CETP expression reduced HDL (21%) and increased non-HDL (15%) fractions. MEST treatment increased the VLDL concentrations (42%) regardless of other interventions. Exercise training reduced triacylglycerol (25%) and free fatty acids (20%), increased both LDL and HDL (25-33%), and reduced CETP (19%) plasma levels. Significant factor interactions showed that the increase in HDL induced by exercise is explained by reducing CETP activity and that MEST blunted the exercise-induced elevation of HDL-cholesterol. These results reinforce the positive metabolic effects of exercise, resolved a controversy about CETP response to exercise and evidenced MEST potency to counteract specific exercise benefits. |
| Databáze: | OpenAIRE |
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