Light microscopic and ultrastructural pathology of seminiferous tubules of rats given multiple doses of Pasteurella multocida group D protein toxin
| Autor: | Richard B. Rimler, Norman F. Cheville, James P. Tappe, J. R. Thurston, Mark R. Ackermann, Dale E. Shuster |
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| Rok vydání: | 1992 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Necrosis Pasteurella multocida 040301 veterinary sciences Bacterial Toxins Spleen Spermatocyte Biology Testicle Toxicology Pathology and Forensic Medicine 0403 veterinary science 03 medical and health sciences Multinucleate Bacterial Proteins Internal medicine Testis medicine Animals Spermatogenesis Molecular Biology Sertoli Cells Histocytochemistry Tumor Necrosis Factor-alpha 04 agricultural and veterinary sciences Cell Biology Organ Size Seminiferous Tubules Sertoli cell Rats Microscopy Electron 030104 developmental biology Endocrinology Seminiferous tubule medicine.anatomical_structure medicine.symptom |
| Zdroj: | Toxicologic pathology. 20(1) |
| ISSN: | 0192-6233 |
| Popis: | Male Holtzman rats were given subcutaneous doses of a purified Pasteurella multocida group D heat-labile toxin on alternate days for up to 22 days. Rats were necropsied at 18 days or 36 days (14 days after last dose of toxin) or when moribund, and testicles were taken for histologic and ultrastructural examination. Other selected tissues, including liver and spleen, were taken for histologic examination. Histologically, testicular and splenic lesions occurred more consistently and at much smaller doses when compared with lesions in other target organs such as liver. Testicular and splenic lesions were present in all rats (6/6) given 0.8 micrograms/kg toxin and were seen in some rats (1/6) given as little as 0.2 micrograms/kg toxin. Only 3/6 rats given 0.8 micrograms/kg toxin had hepatic lesions; no hepatic lesions were seen at doses of 0.2 micrograms/kg. Testicles from toxin-treated rats were smaller and weighed less than controls. Seminiferous tubules were moderately dilated and lined by polygonal sertoli cells. The normal spermatogenic maturation sequence and mature spermatids were absent, and many tubules contained multinucleate spermatocytes. Severely affected tubules were necrotic and mineralized. Ultrastructurally, there was necrosis of adluminal spermatocytes, multinucleate cell formation, and spaces between Sertoli cell plasma membranes. Testicular lesions were similar to those described for vitamin D-deficient rats, vitamin A-deficient rats, vasectomized rats, and rats given intravenous tumor necrosis factor; however, rats given lethal doses of toxin did not have elevated levels of TNF alpha activity. |
| Databáze: | OpenAIRE |
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