Identification of HIV Type 1 Group N Infections in a Husband and Wife in Cameroon: Viral Genome Sequences Provide Evidence for Horizontal Transmission
| Autor: | Catherine A. Brennan, Ruthie Coffey, Pierre Bodelle, Gerald Schochetman, Nicaise Ndembi, Sushil G. Devare, Ana Vallari, Dora Mbanya, Charlotte Ngansop, Lazare Kaptue, Lutz G. Gürtler, Julie Yamaguchi |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Immunology HIV Infections Genome Viral HIV Envelope Protein gp120 Polymerase Chain Reaction Virus law.invention Acquired immunodeficiency syndrome (AIDS) law Virology Disease Transmission Infectious medicine Humans Cameroon Serotyping Spouses Sida Polymerase chain reaction Genetics biology Immunodominant Epitopes Middle Aged biology.organism_classification medicine.disease Peptide Fragments Infectious Diseases Spouse Lentivirus HIV-1 Female Viral disease Horizontal transmission |
| Zdroj: | AIDS Research and Human Retroviruses. 22:83-92 |
| ISSN: | 1931-8405 0889-2229 |
| Popis: | HIV-1 is classified into three groups, M (major), N (non-M non-O), and O (outlier); each group arose from a separate transmission of SIVcpz into humans. HIV-1 group N was recently discovered and infections with this virus are rare with only eight documented cases. All group N infections have been found in Cameroon and there is no evidence of direct linkage between the infected patients. We report here the identification of HIV-1 group N infections in a husband and wife. The group N infection in the husband, 1131-03, was identified first based on seroreactivity in peptide EIAs and confirmed by PCR amplification of group N viral sequences. Subsequently the wife, 1015-04, was evaluated and confirmed to also be infected with a group N virus. Near full-length viral genomes were amplified and sequenced from each patient's specimen. The low level of diversity between the two viral sequences provides evidence of horizontal transmission of group N from one spouse to the other. Patient 1131-03 was receiving antiviral therapy consisting of reverse transcriptase inhibitors; the treatment appears effective for suppression of group N viral replication based on apparently low viral load in plasma specimens collected from the patient and the absence of drug resistance mutations in RT sequences amplified from 1131-03. This report brings to 10 the number of group N infections identified and to 5 the number of group N genomes sequenced. Although group N infections continue to be rare, group N is a pathogenic virus and its prevalence needs to be monitored. |
| Databáze: | OpenAIRE |
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