Negative Control of the Myc Protein by the Stress-Responsive Kinase Pak2
| Autor: | Jolinda A. Traugh, J. Michael Bishop, Zhongdong Huang |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Transcriptional Activation
Tumor suppressor gene Apoptosis Protein Serine-Threonine Kinases Biology Proto-Oncogene Mas DNA-binding protein Cell Line Proto-Oncogene Proteins c-myc Mice Transcription (biology) Animals Humans Phosphorylation Cell Growth and Development Molecular Biology Transcription factor Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Kinase Tumor Suppressor Proteins Cell Cycle DNA Cell Biology Cell cycle Molecular biology Protein Structure Tertiary DNA-Binding Proteins Basic-Leucine Zipper Transcription Factors Cell Transformation Neoplastic p21-Activated Kinases NIH 3T3 Cells Protein Binding Transcription Factors |
| Zdroj: | Molecular and Cellular Biology. 24:1582-1594 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.24.4.1582-1594.2004 |
| Popis: | Pak2 is a serine/threonine kinase that participates in the cellular response to stress. Among the potential substrates for Pak2 is the protein Myc, encoded by the proto-oncogene MYC. Here we demonstrate that Pak2 phosphorylates Myc at three sites (T358, S373, and T400) and affects Myc functions both in vitro and in vivo. Phosphorylation at all three residues reduces the binding of Myc to DNA, either by blocking the requisite dimerization with Max (through phosphorylation at S373 and T400) or by interfering directly with binding to DNA (through phosphorylation at T358). Phosphorylation by Pak2 inhibits the ability of Myc to activate transcription, to sustain cellular proliferation, to transform NIH 3T3 cells in culture, and to elicit apoptosis on serum withdrawal. These results indicate that Pak2 is a negative regulator of Myc, suggest that inhibition of Myc plays a role in the cellular response to stress, and raise the possibility that Pak2 may be the product of a tumor suppressor gene. |
| Databáze: | OpenAIRE |
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