Effect of leptin on in vivo goat embryo production
| Autor: | André Mariano Batista, P L J Monteiro, Maria Madalena Pessoa Guerra, C C D Carvalho, W. A. Gomes, Gustavo Ferrer Carneiro, F. L. M. Silva, Pierre Castro Soares, F. C. Almeida |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Leptin
medicine.medical_specialty medicine.medical_treatment Superovulation DNA Fragmentation Biology Endocrinology In vivo Pregnancy Internal medicine medicine In Situ Nick-End Labeling Animals Humans Blastocyst Saline Progesterone Estrous cycle Estradiol Goats Ovary Embryo Embryo Transfer Recombinant Proteins medicine.anatomical_structure embryonic structures Animal Science and Zoology Female Steroids Follicle Stimulating Hormone Embryo quality Biotechnology Hormone |
| Zdroj: | Reproduction in domestic animals = Zuchthygiene. 49(3) |
| ISSN: | 1439-0531 |
| Popis: | Contents The aim of this study was to evaluate the effect of leptin administration during superovulation on in vivo goat embryo production. Ten mature does were superovulated with 133 mg follicle-stimulating hormone (FSH) i.m. in six descending doses at 12-h intervals. The goats received 4.8 μg/kg human recombinant leptin s.c. (leptin group, n = 5) or phosphate-buffered saline (PBS) (control group, n = 5) with the first and second FSH doses. The does were mated and subjected to embryo collection by transcervical technique 6 days later. The total number of cells per embryo and the number of cells with fragmented DNA were assessed in selected blastocysts by combining Hoechst 33342 and terminal dUTP nick-end labelling (TUNEL) staining. Plasma concentrations of oestradiol (E2) and progesterone (P4) were determined by electrochemiluminescence from the day of FSH treatment, on the day of superovulatory oestrus and on the day before embryo collection. Compared with the control group, the does that received leptin had a higher number of transferable embryos (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text