Human-Induced Neural and Mesenchymal Stem Cell Therapy Combined with a Curcumin Nanoconjugate as a Spinal Cord Injury Treatment

Autor: Miguel A González-Pérez, Hoda Elkhenany, Pablo Bonilla, Michael J. Edel, Ana Alastrue-Agudo, Xavier Navarro, Victoria Moreno-Manzano, María J. Vicent, Esther Giraldo, Joaquim Hernández
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Polymers
polymer-drug conjugate
Nanoconjugates
Pharmacology
Rats
Sprague-Dawley
Acetals
Neural Stem Cells
curcumin
Biology (General)
Induced pluripotent stem cell
Spinal cord injury
Spectroscopy
Cells
Cultured
Microglia
General Medicine
Neural stem cell
polymer–drug conjugate
Computer Science Applications
Chemistry
medicine.anatomical_structure
Neuroprotective Agents
Female
neuroprotection
Stem cell
QH301-705.5
Induced Pluripotent Stem Cells
Mesenchymal Stem Cell Transplantation
Neuroprotection
Catalysis
Article
Inorganic Chemistry
stem cells
medicine
Animals
Humans
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Spinal Cord Injuries
business.industry
Organic Chemistry
Mesenchymal stem cell
Mesenchymal Stem Cells
Recovery of Function
medicine.disease
spinal cord injury
Rats
Transplantation
nervous system
business
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 11
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
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Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
Centro de Investigación Principe Felipe (CIPF)
International Journal of Molecular Sciences, Vol 22, Iss 5966, p 5966 (2021)
ISSN: 1422-0067
2017-2230
DOI: 10.3390/ijms22115966
Popis: Altres ajuts: Fundació Marató TV3 2017/refs.20172230, 20172231 i 20172110 We currently lack effective treatments for the devastating loss of neural function associated with spinal cord injury (SCI). In this study, we evaluated a combination therapy comprising human neural stem cells derived from induced pluripotent stem cells (iPSC-NSC), human mesen-chymal stem cells (MSC), and a pH-responsive polyacetal-curcumin nanoconjugate (PA-C) that al-lows the sustained release of curcumin. In vitro analysis demonstrated that PA-C treatment pro-tected iPSC-NSC from oxidative damage in vitro, while MSC co-culture prevented lipopolysaccha-ride-induced activation of nuclear factor-κB (NF-κB) in iPSC-NSC. Then, we evaluated the combination of PA-C delivery into the intrathecal space in a rat model of contusive SCI with stem cell transplantation. While we failed to observe significant improvements in locomotor function (BBB scale) in treated animals, histological analysis revealed that PA-C-treated or PA-C and iPSC-NSC + MSC-treated animals displayed significantly smaller scars, while PA-C and iPSC-NSC + MSC treatment induced the preservation of β-III Tubulin-positive axons. iPSC-NSC + MSC transplantation fostered the preservation of motoneurons and myelinated tracts, while PA-C treatment polarized microglia into an anti-inflammatory phenotype. Overall, the combination of stem cell transplantation and PA-C treatment confers higher neuroprotective effects compared to individual treatments.
Databáze: OpenAIRE
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