The usefulness of a rapid PCR methodology to detect rearranged Ig heavy chain genes in lymphoproliferative disease in a diagnostic setting
| Autor: | Sumitra Kamath, Rudy I. Angulo, Mark Tschuchnigg, Bruce Bennetts, Elizabeth M. Benson |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Immunoglobulin gene
Time Factors Cost Control Molecular Sequence Data Gene Rearrangement B-Lymphocyte Heavy Chain Biology Polymerase Chain Reaction Sensitivity and Specificity Pathology and Forensic Medicine law.invention chemistry.chemical_compound law Humans Gene Polymerase chain reaction Southern blot Base Sequence DNA Neoplasm Gene rearrangement Molecular biology Lymphoproliferative Disorders Blot Blotting Southern chemistry Immunology biology.protein Antibody DNA |
| Zdroj: | Pathology. 27:352-357 |
| ISSN: | 0031-3025 |
| DOI: | 10.1080/00313029500169293 |
| Popis: | Polymerase chain reaction (PCR) was used to amplify the DNA fragments of the framework 3 region (FR3) of the immunoglobulin heavy (IgH) chain genes, from the tissue of 66 patients with B-lymphoproliferative diseases and 74 patients with other malignant diseases, reactive or normal tissue. The assay performed with 77% sensitivity, 100% specificity and 89% efficacy. In addition, the PCR assay cost less than 25% of the cost performing Southern blot analysis of tumor DNA, which has been the test performed to date, and had a turn around time of 24 hrs rather than the 7-14 days required to obtain a result from Southern blot analysis. These results suggest that PCR analysis of B-cell lymphoproliferative disease is superior to Southern blot analysis, in the setting of a diagnostic laboratory. |
| Databáze: | OpenAIRE |
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