Rapid detection of Mycobacterium avium in stool samples from AIDS patients by immunomagnetic PCR
| Autor: | P Marino, G H Bai, Z M Li, Sheldon L. Morris, M J Brennan, N Gine, C F von Reyn |
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| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Bacilli Time Factors medicine.drug_class Mycobacterium avium-intracellulare infection Antimycobacterial Immunomagnetic separation Monoclonal antibody Polymerase Chain Reaction Sensitivity and Specificity law.invention Microbiology Feces law medicine Humans Polymerase chain reaction DNA Primers Mycobacterium avium-intracellulare Infection Acquired Immunodeficiency Syndrome biology Immunomagnetic Separation bacterial infections and mycoses 16S ribosomal RNA biology.organism_classification medicine.disease Virology Research Article Mycobacterium |
| Zdroj: | Journal of Clinical Microbiology. 34:1903-1907 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Direct PCR detection of bacteria in clinical samples is often hindered by the presence of compounds that inhibit the PCR. To improve and accelerate the diagnosis of Mycobacterium avium-M. intracellulare complex infections, an immunomagnetic PCR (IM-PCR) assay was developed. This IM-PCR procedure combines the separation of mycobacteria by antimycobacterial monoclonal antibody coupled to magnetic beads with an M. avium-M. intracellulare complex-specific PCR protocol based on 16S rRNA gene sequences. As few as 10 M. avium bacilli were detected in spiked human stool samples, a clinical specimen usually refractory to conventional PCR analysis, by the IM-PCR method. Moreover, M. avium organisms were detected in about 24 h in 18 of 22 culture-confirmed fecal samples from AIDS patients. This IM-PCR protocol should allow for the rapid and sensitive detection of M. avium isolates in clinical specimens. |
| Databáze: | OpenAIRE |
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